0:00
Hello.
My name is Dr. Alan Pittman,
from the Institute of Neurology.
Today I'm going to be
talking about next generation
sequencing in genetic diagnostics.
0:12
So just to give you a quick
outline of today's lecture,
first we'll be talking about
Sanger sequencing methods.
I'm then going to move on to next
generation sequencing technologies.
I'm going to talk about
target enrichment, how we
analyze next generation
sequencing data.
And then I'm going to
talk about applications
of this technology in
genetic diagnostics.
0:33
First up, Sanger sequencing.
0:37
Sanger sequencing.
This technique was first invented
by Fred Sanger back in 1977.
It's a cycle sequencing
technique based
on the principle of full
color chain terminators.
This method is extremely
accurate but very slow.
0:55
The Human Genome Project.
This project began back in 1990
and took 13 years to complete.
The 3 billion base pair
long genome was all
sequenced with the Sanger method.
The reason this was undertaken
was to drive genetics research.
We can now achieve this amount of
sequencing in as little time as one
day with the new technologies
I will be talking to you about.
1:22
Moving on to next generation
sequencing technologies.
1:27
The next generation
of DNA sequencing.
So recently we have moved
on from Sanger sequencing
to a more high-throughput
approach, the biggest change
being now we sequence
DNA molecules in parallel
rather than one sequence at a time.
This has been driven by new
technological advances which I will
be speaking about through
the remainder of the lecture.
