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The following session we'll
cover protein purification,
including the recombinant
expression of proteins and
the choice of expression systems
based on specific needs.
We'll discuss the main steps
in protein recovery and
the techniques used to purify
proteins by exploiting
their unique properties,
such as chromatography methods.
Additionally, we'll
explore how to
monitor the yield, purity,
and identity of
proteins throughout
the process using
analytical techniques.
Finally, we will
address the challenges
and strategies for scaling up
purification processes
to industrial levels
while maintaining product
quality and compliance.
Protein purification is
central in biotechnology,
enabling isolation of
target proteins from
complex mixtures for research,
diagnostics or therapy.
Most proteins today are
produced recombinantly.
The gene is cloned and over
expressed in an
expression system.
Bacteria like E coli
are widely used
as they're easy to culture
and yield abundant protein.
Yeast, insect, and
mammalian systems
are chosen when
post translational
modifications are needed.
The choice of system
is determined
by the protein's properties
and application.
After recombinant
expression, the next step
is protein recovery through
downstream processing,
starting with cell lysis,
where cells are
disrupted by mechanical,
chemical, or enzymatic means.
The lysate is clarified by
centrifugation or filtration
to remove debris.
Proteins, along
with nucleic acids,
lipids and carbohydrates,
remain in a complex mixture.
