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1. Introduction
2. Cocaine
3. Coca-leaf
4. Crop spraying
5. Cocaine was often used in commercial products
6. Transition to addiction
7. Cocaine action
8. Cocain action in the synapse
9. 4 drugs: 1 final destination
10. The brain areas involved in addiction
11. Cocaine addiction is genetic
12. Twin studies
13. The dopamine transporter gene (SLC6A3)
14. Details of the exon/intron structure of DAT1
15. Structure of LD across DAT1
16. The dopamine transporter gene
17. Variable number tandem repeats/microsatellites
18. Brazilian cocaine association sample
19. Study design
20. DAT intron 8 VNTR
21. 30bp VNTR
22. Introns 8 alleles in cocaine cases and controls
23. Introns 8 genotypes in cocaine cases and controls
24. Association due to another variation in the gene
25. The most obvious confounder
26. Brazilian population is genetically heterogeneous
27. Stratification analysis
28. The subpopulations form stratification analysis
29. Is the VNTR functional?
30. Reporter gene construct
31. The expression experiment
32. Differential expression of intron 8 VNTR alleles
33. Summary
34. Amphetamine and methamphetamine
35. Amphetamine
36. Methamphetamine
37. Methamphetamine ice
38. Action of amphetamine and methamphetamine
39. Amphetamine effects on dopamine neurons
40. Subjects for case control study
41. Marker selection
42. Genotype frequencies in cases and control
43. Association analyses
44. Haplotype analyses
45. The expression experiment
46. Reporter gene construct
47. Preliminary functional work
48. Conclusions
49. Intron 8 is also associated with ADHD
50. Transmission ratios from parents to offspring
51. Observations
52. Acknowledgements

Topics Covered

• Heritability of cocaine and other addictions
• Gene-environment interactions and addiction
• The Dopamine transporter gene (SLC6A3)
• Brazilian Cocaine Association sample
• Case-control cohort from Sao Paulo, Brazil
• Association between the variants and cocaine addiction
• Potential study confounders
• Correcting for population stratification
• Reporter-gene assays indicating that the VNTR is a differential response element
• Functional assay of associated genetic variation
• Different expression levels of the alleles
• Allele and genotype association between DAT-intron 8 VNTR and cocaine
• Similar studies on amphetamine and methamphetamine addiction

Links

Series:

• Psychopharmacogenetics

Categories:

• Genetics & Epigenetics
• Neuroscience

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Gerome Breen has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.