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- Introduction
-
1. Drosophila genetics - the first 25 years
- Prof. Dan Lindsley
- Establishment of the Primary Body Axes
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2. Homeotic genes in Drosophila's bithorax complex - The legacy of Ed Lewis
- Prof. Francois Karch
- Cell Type Specification and Organ Systems
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4. From germ cell specification to gonad formation
- Prof. Ruth Lehmann
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5. Drosophila stem cells
- Prof. Michael Buszczak
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6. Legacy of drosophila genetics: female germline stem cells
- Prof. Michael Buszczak
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7. Intestinal stem cell-mediated repair in Drosophila 1
- Prof. Tony Ip
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8. Intestinal stem cell-mediated repair in Drosophila 2
- Prof. Tony Ip
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10. Axon guidance in Drosophila
- Prof. John Thomas
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11. Development and physiology of the heart
- Prof. Rolf Bodmer
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12. Identification of host defenses in the Drosophila gut using genome-scale RNAi
- Prof. Dominique Ferrandon
- Genome Organization and Function
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13. The genetic analysis of meiosis in Drosophila melanogaster females
- Prof. R. Scott Hawley
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15. Dorsal-ventral patterning of the Drosophila embryo
- Prof. Mike Levine
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17. Genome-wide pooled CRISPR screen in arthropod cells
- Prof. Norbert Perrimon
- Behavior
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19. Genetics of chemosensory transduction: taste and smell
- Dr. Leslie Vosshall
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20. Cracking the case of circadian rhythms by Drosophila genetics
- Prof. Jeffrey C. Hall
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21. Sleep in Drosophila
- Dr. Ralph Greenspan
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23. Drosophila as a model for drug addiction
- Prof. Ulrike Heberlein
- Mechanism of Human Disease
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24. Cross-genomic analysis of human disease genes
- Prof. Ethan Bier
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25. Human neurodegenerative disease: insights from Drosophila genetics
- Prof. Nancy Bonini
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26. Metastasis of Drosophila tumors
- Prof. Allen Shearn
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27. Rac-enhanced CAR immunotherapy: RaceCAR
- Prof. Denise Montell
- Evolution of Adaptive Novelties
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29. The evolution of morphological novelty
- Prof. Nipam Patel
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30. The genetic architecture of complex traits: lessons from Drosophila
- Prof. Trudy Mackay
- Archived Lectures *These may not cover the latest advances in the field
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31. Using gene expression information to provide insights into patterning and differentiation
- Prof. Angelike Stathopoulos
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32. Regulation of gastrulation in Drosophila
- Prof. Dr. Maria Leptin
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33. microRNA function in stem cells
- Prof. Hannele Ruohola-Baker
Printable Handouts
Navigable Slide Index
- Introduction
- Why study Drosophila immunity?
- Phylogeny of host defenses
- A world of microbes
- Responses to a septic injury
- The host defense against intestinal infections
- The Drosophila digestive tract
- The oral infection model
- Drosophila intestinal barriers
- Chemical barriers in intestinal host defense
- imd protects against intestinal infections
- Tolerance to microbiota & the IMD pathway (1)
- Tolerance to microbiota & the IMD pathway (2)
- Serratia marcescens
- Serratia Db11 and the humoral immune response
- A model of oral infection
- What happens to Serratia in the hemocoel?
- Phagocytosis in Drosophila host defense
- Host defenses against ingested S. marcescens
- S. marcescens damages the midgut epithelium
- Intestinal stem cells in infected guts
- A genome-wide RNAi screen
- Screening mutant lines
- The Gal4-UAS system
- Transgenic RNAi in Drosophila
- RNAi induction-TARGET system
- Scheme of the genome-wide screen
- Pros and cons of the RNAi strategy
- RNAi primary screen in numbers
- Gene ontology of susceptible hits
- Gene ontology of resistant hits
- Secondary screen
- Statistically enriched cellular processes
- The JAK/STAT pathway in Drosophila
- UPD and UPD3 induced during Serratia infection
- 10xSTAT-GFP reporter
- JAK/STAT pathway activation
- JAK-STAT signaling in stem cells
- JAK-STAT pathway and proliferation of ISCs
- Homeostasis in midgut during intestinal infection
- Host defense against microbial infections
- Features of endurance/tolerance
- Host defenses in the Drosophila intestine
- Endurance during the early phase of infection
- Time course of infection
- Serratia hemolysin is a pore forming toxin
- Hemolysin’s role in the early phase
- E. coli transformed with hemolysin
- Increased intracellular vacuolation by hemolysin
- High magnification of a vacuole
- The early phase phenotype after Db11 infection
- Organelles are affected during the early phase
- Partial recovery at 24 hours
- A mutant involved in endurance of early phase
- Endurance against pore-forming toxins?
- Take-home message
- Acknowledgements (1)
- Acknowledgements (2)
Topics Covered
- Why use drosophila to study immunity?
- Drosophila digestive tract
- Intestinal barriers against pathogens
- The imd pathway in preventing infection
- Serratia marcescens, an opportunistic pathogen
- Genome wide RNA-I screening to identify essential genes for host defense
- Gene ontology of the hits from RNA-I screening
- The JAK/STAT pathway
- The components of host defenses to infections
- Serratia hemolysins
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Ferrandon, D. (2014, February 4). Identification of host defenses in the Drosophila gut using genome-scale RNAi [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/EGGC1930.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Dominique Ferrandon has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Identification of host defenses in the Drosophila gut using genome-scale RNAi
Published on February 4, 2014
37 min
A selection of talks on Immunology & Inflammation
Transcript
Please wait while the transcript is being prepared...
0:00
Hello
My name is Dominique Ferrandon.
I've been working on
Drosophila innate immunity
in Strasbourg for
more than 15 years.
Today, I'm going to present how Drosophila are used
to model intestinal infections
and shows that, thanks
to its advanced genetics, it
provides a powerful paradigm
to understand the different
facets of mucous host defenses.
0:24
The first question
to address is- why
one should use Drosophila
to study innate immunity?
Many fly stocks can be raised
at relatively low cost.
Also Drosophila has a short life cycle
and produces an abundant offspring.
As will be illustrated in
this talk, its main strength,
however, lies in its genetics,
especially the ability
to perform genome-wide
screens in a living animal.
As regards immunity, flies like
a conventional adaptive immunity.
And first, it is easier to
correlate the effect of mutations
in immunity genes to a phenotype of
susceptibility to a given pathogen.
1:01
This slide shows that we share with
Drosophila a common ancestor that
lived more than three-quarters
of a billion years ago.
One should note the
acquisition of the machinery
to generate somatic recombinant immunoreceptors in gnathosomes.
And first, you must
extend vertebrates
some 450 million years ago.
Thus, given this long
period of evolution,
is it meaningful to study
the anatomy of insects
to understand our own immune system?
1:28
The answer to this question
is illustrated in this slide.
This common Metazoan
ancestor evolved
in a world that was already
dominated by microbes.
Thus, evolution had already selected
a primeval immune system from which
both the protostomian and the
deuterostomian immune systems
are derived.
Even though they may not
share the same molecules,
many of the primordial
principles have been conserved
throughout evolution, and therefore
results obtained in the Drosophila
model system may guide
investigations in mammalian models.
This concept is especially
well-illustrated by the study
of the Drosophila
systemic immune response
presented in the next slide.
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