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1. Introduction
2. Complex protein interactions in local domains
3. Cellular reactions in Ca2+- ‘nanodomains‘
4. Analysis of protein nano-environments
5. Experimental approach: mouse & rat brain
6. Experimental approach: solubilization
7. Experimental approach: affinity purification
8. Experimental approach: MS analysis
9. Quantification and evaluation (1)
10. Quantification and evaluation (2)
11. Nano-environments of Cav2 channels
12. Details and ‘settings‘ of the proteomic approach
13. Nano-environment: the channels‘ core
14. Nano-environment: identified protein constituents
15. Nano-environment circuitry
16. Molecular model of Cav2 nano-environment (1)
17. Molecular model of Cav2 nano-environment (2)
18. Molecular model of Cav2 nano-environment (3)
19. BKCa-Cav2 channels complexes (1)
20. BKCa-Cav2 channels complexes (2)
21. BKCa-Cav2.1 complexes in X. oocytes
22. BKCa-Cav2.1: a prototypic ‘Ca2+ nano-domain‘
23. Summary and conclusions
24. Acknowledgements

Topics Covered

• Significance and molecular organization of local Ca2+ signalling
• Proteomic analysis of the nano-environment of voltage-gated Ca2+ (Cav) channels
• Structure and operation of the Cav2 channels from mammalian brain
• Operation of Cav-BK channel complexes
• Cav-BK complex as a prototypic Ca2+ nano-domain

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Neurology

Talk Citation

Fakler, B. (2013, October 7). Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/DLAD1050.
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Publication History

• Published on October 7, 2013

Financial Disclosures

• Prof. Bernd Fakler has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.