• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 20 min
  2. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 30 min
  3. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  4. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  5. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 34 min
  6. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 37 min
  7. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 60 min
  8. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 35 min
  9. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 47 min
  10. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 53 min
  11. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 31 min
  12. Molecular biology of ryanodine receptors

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• 57 min
  13. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 50 min
  14. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 43 min
  15. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• Calcium Efflux and Sequestration
• 45 min
  16. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  17. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 45 min
  18. Sodium-calcium exchange

  • Prof. John Reeves
• 22 min
  19. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• Organelle Calcium
• 42 min
  20. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 54 min
  21. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• 31 min
  22. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 34 min
  23. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  24. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• Spatiotemporal Calcium Signals
• 58 min
  25. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 54 min
  26. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 26 min
  27. Modeling Ca2+ signals

  • Dr. David Friel
• Calcium Effectors
• 34 min
  28. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 41 min
  29. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 32 min
  30. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  31. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  32. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  33. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  34. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  35. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  36. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 43 min
  37. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 28 min
  38. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• 38 min
  39. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  40. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks
• 46 min
  41. Genetic defects and calcium

  • Prof. Tullio Pozzan
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  42. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  43. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  44. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  45. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  46. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  47. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Higher order physiological processes
3. An example of the issue
4. Further elaboration of the issue
5. Advantages of genetically encoded sensors
6. Challenges
7. Molecular scale measurements in mammals
8. Circular permutation of GFP
9. GCaMP2
10. Calcium alters GCaMP fluorescence
11. GCaMP Ca2+ fluorescence
12. cmGCaMP2 mice
13. In vivo cardiac imaging (1)
14. In vivo cardiac imaging (2)
15. First in vivo measure of cytosolic Ca2+ in the heart
16. In vivo cardiac imaging (3)
17. Biochemical characterization in cardiac cytosol
18. Kinetic limitations
19. In vivo physiology
20. Cardiac development
21. Pre-septated: embryonic day 10.5
22. Embryonic cardiac cycle
23. Premature ventricular activation
24. Embryonic day 13.5
25. Embryonic day 13.5 imaging
26. Mapping activation in vivo
27. Direct surface conduction - developmental period
28. Cx40 expression
29. Cx40BACGCaMP2
30. Physiology learned (1)
31. Cardiac cell based therapy
32. Arrhythmia vulnerability
33. Embryonic cardiomyocytes
34. Paradigm
35. Conduction block
36. Exogenous cells can electrically couple (1)
37. Exogenous cells can electrically couple (2)
38. Physiology learned (2)
39. GCaMP2 ES cells (1)
40. Endothelial cells
41. Cx40BAC-GCaMP2
42. Cremaster arteriole in vivo
43. Arterial diameter control by endothelial cell Ca2+
44. Highly localized nature of the release sites
45. Physiology learned (3)
46. The future
47. Acknowledgements

Topics Covered

• Calcium Signaling
• How to study complex biology
• Genetically encoded Ca2+ indicators
• GcaMPs
• Structural basis of Ca2+ sensing
• Lineage specification of indicators in transgenic mice
• Real-time cardiac activation
• Response time considerations
• Heart development
• Cell based therapy and coupling
• Embryonic stem cells and GCaMPs
• Vascular control and pulsars
• Future directions

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Publication History

• Published on October 7, 2013
• Archived on August 13, 2020

Financial Disclosures

• Dr. Michael I. Kotlikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.