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Navigable Slide Index

1. Introduction
2. Higher order physiological processes
3. An example of the issue
4. Further elaboration of the issue
5. Advantages of genetically encoded sensors
6. Challenges
7. Molecular scale measurements in mammals
8. Circular permutation of GFP
9. GCaMP2
10. Calcium alters GCaMP fluorescence
11. GCaMP Ca2+ fluorescence
12. cmGCaMP2 mice
13. In vivo cardiac imaging (1)
14. In vivo cardiac imaging (2)
15. First in vivo measure of cytosolic Ca2+ in the heart
16. In vivo cardiac imaging (3)
17. Biochemical characterization in cardiac cytosol
18. Kinetic limitations
19. In vivo physiology
20. Cardiac development
21. Pre-septated: embryonic day 10.5
22. Embryonic cardiac cycle
23. Premature ventricular activation
24. Embryonic day 13.5
25. Embryonic day 13.5 imaging
26. Mapping activation in vivo
27. Direct surface conduction - developmental period
28. Cx40 expression
29. Cx40BACGCaMP2
30. Physiology learned (1)
31. Cardiac cell based therapy
32. Arrhythmia vulnerability
33. Embryonic cardiomyocytes
34. Paradigm
35. Conduction block
36. Exogenous cells can electrically couple (1)
37. Exogenous cells can electrically couple (2)
38. Physiology learned (2)
39. GCaMP2 ES cells (1)
40. Endothelial cells
41. Cx40BAC-GCaMP2
42. Cremaster arteriole in vivo
43. Arterial diameter control by endothelial cell Ca2+
44. Highly localized nature of the release sites
45. Physiology learned (3)
46. The future
47. Acknowledgements

Topics Covered

• Calcium Signaling
• How to study complex biology
• Genetically encoded Ca2+ indicators
• GcaMPs
• Structural basis of Ca2+ sensing
• Lineage specification of indicators in transgenic mice
• Real-time cardiac activation
• Response time considerations
• Heart development
• Cell based therapy and coupling
• Embryonic stem cells and GCaMPs
• Vascular control and pulsars
• Future directions

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Kotlikoff, M.I. (2013, October 7). Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/2629/.
Export Citation (RIS)

Publication History

• Published on October 7, 2013
• Archived on August 13, 2020

Financial Disclosures

• Dr. Michael I. Kotlikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.