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Printable Handouts
Navigable Slide Index
- Introduction
- Higher order physiological processes
- An example of the issue
- Further elaboration of the issue
- Advantages of genetically encoded sensors
- Challenges
- Molecular scale measurements in mammals
- Circular permutation of GFP
- GCaMP2
- Calcium alters GCaMP fluorescence
- GCaMP Ca2+ fluorescence
- cmGCaMP2 mice
- In vivo cardiac imaging (1)
- In vivo cardiac imaging (2)
- First in vivo measure of cytosolic Ca2+ in the heart
- In vivo cardiac imaging (3)
- Biochemical characterization in cardiac cytosol
- Kinetic limitations
- In vivo physiology
- Cardiac development
- Pre-septated: embryonic day 10.5
- Embryonic cardiac cycle
- Premature ventricular activation
- Embryonic day 13.5
- Embryonic day 13.5 imaging
- Mapping activation in vivo
- Direct surface conduction - developmental period
- Cx40 expression
- Cx40BACGCaMP2
- Physiology learned (1)
- Cardiac cell based therapy
- Arrhythmia vulnerability
- Embryonic cardiomyocytes
- Paradigm
- Conduction block
- Exogenous cells can electrically couple (1)
- Exogenous cells can electrically couple (2)
- Physiology learned (2)
- GCaMP2 ES cells (1)
- Endothelial cells
- Cx40BAC-GCaMP2
- Cremaster arteriole in vivo
- Arterial diameter control by endothelial cell Ca2+
- Highly localized nature of the release sites
- Physiology learned (3)
- The future
- Acknowledgements
Topics Covered
- Calcium Signaling
- How to study complex biology
- Genetically encoded Ca2+ indicators
- GcaMPs
- Structural basis of Ca2+ sensing
- Lineage specification of indicators in transgenic mice
- Real-time cardiac activation
- Response time considerations
- Heart development
- Cell based therapy and coupling
- Embryonic stem cells and GCaMPs
- Vascular control and pulsars
- Future directions
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Talk Citation
Kotlikoff, M.I. (2013, October 7). Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/GPQQ9950.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Michael I. Kotlikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo
A selection of talks on Biochemistry
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