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1. Introduction
2. Glycosyltransferases (1)
3. Donors used by glycosyltransferases (1)
4. Donors used by glycosyltransferases (2)
5. Nucleotide donors
6. Lipid donors for glycosyltransferases
7. Acceptors used by glycosyltransferases
8. Glycosyltransferase reactions
9. Typical mammalian cell surface oligosaccharides
10. Diversity of sugars (1)
11. Diversity of sugars (2)
12. Diversity of sugars (3)
13. Blood group A and B structures
14. Glycosyltransferases (2)
15. Structural folds for nucleotide glycosyltransferases
16. Model enzymes for glycosyltransferases
17. History of blood group ABO (1)
18. History of blood group ABO (2)
19. History of blood group ABO (3)
20. History of blood group ABO (4)
21. History of blood group ABO (5)
22. Glycosyltransferases GTA/GTB
23. Blood group biochemistry and genetics
24. Transmembrane structure and cloning
25. Expression and crystallography trials
26. GTA and GTB crystals
27. GTA and GTB structure
28. Findings from 2002
29. 3-deoxy-gal inhibitor
30. Ordering of previously disordered structures
31. Enzyme structure at substrate binding
32. Inhibitors: interference with loop closure
33. Electron density for intact donor at active site
34. Excellent agreement with NMR conformation
35. Comparison with other glycosyltransferases
36. Recognition of UDP-gal by GTB: deoxy mapping
37. Conclusions so far
38. Mechanistic insights (1)
39. What is the basis for donor specificity
40. Only 2 of 4 critical residues in binding site (1)
41. Only 2 of 4 critical residues in binding site (2)
42. Which amino acid determines donor specificity?
43. Mechanistic insights (2)
44. Natural mutants identified in blood banks
45. 11 of 200 mutations in blood bank screens
46. P156L mutants: GTB no effect on kinetics
47. P156L mutants: GTA activity reduced by half
48. R176G BAAA: "super A" 4 X increase
49. Single point mutation P234S
50. M214R GTB near DXD metal binding motif
51. Ten new mutations/new hot spots?
52. Insights from natural mutants
53. Acknowledgements
54. Collaborations

Topics Covered

• Reactions catalyzed by glycosyltransferases: substrates and products
• Glycosyltransferase structural folds
• History of Blood group A and B enzymes as models for glycosyltransferase structures and mechanisms
• Properties of natural and unnatural blood group A and B glycosyltransferase mutants

Links

Series:

• Glycobiology

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Haematology

Talk Citation

Publication History

• Published on November 27, 2012
• Archived on July 5, 2022

Financial Disclosures

• Prof. Monica Palcic has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.