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1. Introduction
2. Importance of molecular chaperones
3. Protein folding process
4. Transitions during protein folding
5. Protein folding in the cytosol
6. Chaperone assisted protein folding: history
7. Protein folding in vitro and in mitochondria
8. Protein folding is often competed by aggregation
9. The cellular environment is highly crowded
10. Chaperone-assisted protein folding in E. coli
11. Reconstitution of GroEL-assisted folding
12. Early electron micrographs of GroEL
13. GroES binds to the ends of the GroEL cylinder
14. GroEL and GroES function as a folding cage
15. GroEL structure
16. Which proteins need GroEL/GroES for folding?
17. Identification of the GroEL interaction proteome
18. Class III substrates are of low cellular abundance
19. Cellular depletion of GroEL/ES
20. Size distribution of class III proteins
21. Enrichment of TIM beta/alpha-barrel fold
22. GroEL substrates have complex structures
23. Chaperonins provide a folding environment
24. GroEL as part of cytosolic chaperone network
25. Cooperation of GroEL with upstream chaperones
26. A summarizing movie
27. Acknowledgements

Topics Covered

• De novo protein folding and proteome maintenance critically depend on molecular chaperones
• Transitions during protein folding
• Chaperone assisted protein folding
• Productive protein folding is often competed by aggregation
• Reconstitution of GroEL-assisted folding
• GroEL and GroES function as a folding cage for proteins up to 60 kDa
• GroEL structure
• Which proteins need GroEL/GroES for folding?
• Identification of the GroEL interaction proteome
• Class III substrates are of relatively low cellular abundance but occupy most of the GroEL capacity
• Chaperonins provide a specialized folding environment with two functional elements: sequestration and steric confinement in a hydrophilic cage
• GroEL as part of the cytosolic chaperone network

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on September 5, 2012

Financial Disclosures

• Prof. Dr. F. Ulrich Hartl has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.