Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Outline
3. Introduction - innate immunity
4. PAMPs/MAMPs
5. DAMPs
6. Pathogen recognition receptors
7. Pattern recognition receptors can trigger ...
8. Toll like receptors
9. PRR families
10. RIG-I like receptors
11. C-type lectin and NLR receptors
12. IL-1-beta maturation by caspase-1
13. Caspase-1 activation
14. Inflammasome working model
15. The human NLR family
16. The inflammasomes
17. Intracellular DNA sensing (1)
18. Intracellular DNA sensing (2)
19. Endosomal DNA recognition
20. Intracellular DNA sensing (3)
21. DNase2 deficiency
22. DNase2 deficiency leads to type I IFN production
23. Intracellular DNA sensing (4)
24. Intracellular dsDNA
25. dsDNA activation of caspase-1 (1)
26. dsDNA activation of caspase-1 (2)
27. DNA inflammasome receptor
28. The PYHIN protein family (1)
29. The PYHIN protein family (2)
30. Only AIM2 is expressed in the cytosol
31. AIM2 inflammasome reconstitution in 293T cells
32. AIM2 binds to dsDNA via its HIN domain
33. Inflammasome activation by intracellular DNA
34. Inflammasome activation by Vaccinia virus
35. AIM2 and Listeria monocytogenes sensing
36. The AIM2 inflammasome (1)
37. The AIM2 inflammasome (2)
38. AIM2 and pro-inflammatory cytokines induction
39. Intracellular DNA triggers type I IFN production (1)
40. Intracellular DNA triggers type I IFN production (2)
41. Intracellular DNA triggers type I IFN production (3)
42. poly(dA:dT) forms B-DNA
43. I: Type I IFN induction by dsDNA
44. II: Type I IFN induction by dsDNA
45. RIG-I and MAVS (IPS1)
46. 5' triphosphate RNA
47. RNA from poly(dA-dT) transfected cells is active
48. What about dsDNA from other sources?
49. There are at least two DNA sensing pathways
50. Poly(dA-dT) induced RNA
51. RNA polymerases
52. RNA Pol III as a possible candidate polymerase
53. RNA pol III
54. KO of RNA pol III
55. Physiological relevance
56. Epstein-barr virus
57. Epstein-barr virus encoded RNAs
58. EBV, model pathogen for innate immune pathways
59. RNA pol III inhibition and EBER transcription
60. RNA pol III inhibition and IFN-alpha production
61. Indirect DNA sensing via an RNA intermediate
62. STING signal is essential for type I IFN induction
63. STING is required for DNA sensing
64. STING-deficient cells
65. STING senses live pathogens
66. Is STING a signalling mediator or a receptor? (1)
67. Is STING a signalling mediator or a receptor? (2)
68. Is STING a signalling mediator or a receptor? (3)
69. c-di-GMP and c-di-AMP are MAMP molecules
70. Is STING a signalling mediator or a receptor? (5)
71. Is there more ...?
72. LRRFIP1
73. Ku70 drives the transcription of IFN-lambda1
74. Concluding remarks
75. STING - master regulator of DNA recognition
76. The RNA Pol III - RIG-I pathway
77. Acknowledgements

Topics Covered

• Mechanisms of intracellular DNA sensing
• Intracellular DNA sensing that results in inflammasome activation
• DNA sensing mechanisms leading to pro-inflammatory gene expression

Links

Series:

• Cells of the Innate Immune System

Categories:

• Biochemistry
• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on June 11, 2012

Financial Disclosures

• Prof. Veit Hornung has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.