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1. Introduction
2. Pathogens\Commensals at the mucosal level
3. Urinary tract infections (UTI)
4. UTI frequency
5. Uropathogenic Escherichia coli virulence
6. Asymptomatic carrier strains
7. Uropathogenic E. coli vs. ABU strains
8. Human UTI- resistance v. susceptibility
9. Host resistance in the murine UTI model
10. Innate immune control of host resistance
11. Innate immune response and effector function
12. How pathogenic bacteria activate a host response
13. Uropathogenic E. coli, attachment and disease
14. P fimbriae and recognition receptors
15. Pathogen specific innate immune signaling (1)
16. TLR4-ceramide interactions defined by FRET
17. Proteomic and transcriptomic analysis
18. Nuclear IRF-3 translocation
19. Importance of identifying signaling pathways
20. Pathogen specific innate immune signaling (2)
21. IRF3 knock out
22. IRF3 -/- phenotype
23. IL8 receptor knock out
24. IL-8 and IL-8R; neutrophils are crucial effector cells
25. Neutrophil exodus across the mucosa
26. mIL-8Rh-/-mice
27. IL-8 receptor phenotype
28. IL-8R KO phenotype
29. Neutrophil entrapment in IL-8R KO mice
30. Neutrophils accumulate in the kidneys
31. mIL-8R KO mice develop renal scarring
32. Acute and chronic pyelonephritis
33. Conclusions, IRF3 and IL-8
34. TLR4 -/- mice
35. Asymptomatic bacteriuria in C3H/HeJ & Tlr4 KO's
36. Conclusion: Loss of TLR4 protects
37. Genetic variance affects UTI progression
38. Genetic susceptibility to UTI
39. Human UTI susceptibility
40. Researching disease-associated polymorphisms
41. IRF3 promoter polymorphisms in UTI patients
42. The SNPs reduce IRF3 promoter activity
43. Clinical studies to identify CXCR1 polymorphisms
44. CXCR1 sequencing
45. SNP1 affects AML1 dependent transcription
46. SNP frequency in APN-prone patients and controls
47. Variant frequency-APN-prone children vs. controls
48. TLR4 - prediction from the mouse model
49. Clinical studies to identify TLR4 polymorphisms
50. Children with ABU have low TLR4 expression
51. Many have looked for mutations in structural genes
52. New TLR4 promoter polymorphisms
53. SNP genotype frequency in children with UTI
54. TLR4 promoter genotype in asymptomatic carriers
55. Low response to infection in reporter assays
56. Summary-TLR4, CXCR1, IRF3 role in UTI response
57. Susceptibility to UTI can be inherited
58. Commensalism vs. Virulence
59. Future consequences for diagnosis and therapy
60. Contributors & Thanks

Topics Covered

• Pathogens\Commensals at the mucosal level
• How pathogenic bacteria activate a host response
• Pathogen specific innate immune signaling
• Urinary tract infections (UTI)
• Uropathogenic E. coli vs. ABU strains
• TLR4, CXCR1, IRF3, IL8R role in UTI response
• Importance of identifying signaling pathways & crucial effector cells
• Acute/Chronic pyelonephritis & asymptomatic bacteriuria
• Genetics & UTI
• Researching disease-associated polymorphisms
• SNPs and their effect in APN & UTI
• Mutations in structural genes
• Response to infection in reporter assays
• Commensalism vs. Virulence

Links

Series:

• Cells of the Innate Immune System

Categories:

• Biochemistry
• Cell Biology
• Immunology

Therapeutic Areas:

• Gynaecology & Obstetrics
• Immunology & Inflammation

Talk Citation

Svanborg, C. (2012, June 11). Innate immunity in the genitourinary tract: genetics of susceptibility to disease or asymptomatic carriage [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 21, 2024, from https://doi.org/10.69645/HUWI6906.
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Publication History

• Published on June 11, 2012

Financial Disclosures

• Prof. Catharina Svanborg has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.