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- Overview of Chaperone Networks
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1. Mapping the molecular chaperone interaction network in yeast
- Prof. Walid A. Houry
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2. The interaction network of the GroEL chaperonin
- Prof. Dr. F. Ulrich Hartl
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3. Human heat shock protein families
- Prof. Herman Kampinga
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4. Extracellular proteostasis: an emerging field
- Prof. Mark Wilson
- Proteasome Networks
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6. Biogenesis of the eukaryotic proteasome
- Prof. Mark Hochstrasser
- Understanding Intrinsically Disordered Proteins in Protein Homeostasis
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7. Intrinsically unstructured proteins: regulation and disease
- Dr. M. Madan Babu
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8. The roles of intrinsic disorder in protein interaction networks
- Prof. Vladimir N. Uversky
- Gene Regulatory Networks and their Role in Protein Homeostasis
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10. Structure, evolution and dynamics of gene regulatory networks
- Dr. M. Madan Babu
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12. Protein-protein interaction networks
- Prof. Peter Csermely
- Protein Homeostasis in the Endoplasmic Reticulum
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13. Role of calnexin and calreticulin in protein homeostasis within the endoplasmic reticulum
- Prof. David B. Williams
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14. The unfolded protein response
- Prof. Kazutoshi Mori
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15. Role of ER stress in cystic fibrosis airway inflammation
- Dr. Carla Maria Pedrosa Ribeiro
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16. The recognition of misfolded glycoproteins in the endoplasmic reticulum
- Dr. David Y. Thomas
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17. Chaperone systems of the endoplasmic reticulum
- Prof. Linda M. Hendershot
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18. The ERAD network
- Prof. Daniel Hebert
- Protein Homeostasis in the Mitochondria
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19. Protein homeostasis in mitochondria: AAA+ chaperones & proteases
- Dr. David A. Dougan
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21. Mitochondrial chaperonin Hsp60: locations, functions and pathology
- Prof. Francesco Cappello
- Prof. Alberto J. L. Macario
- Protein Homeostasis in the Nucleus
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22. Nuclear protein quality control degradation
- Dr. Richard G. Gardner
- Protein Homeostasis in Aging Disease
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23. Protein homeostasis during ageing: C. elegans as a model organism
- Prof. Nektarios Tavernarakis
- Protein Homeostasis in Neurodegeneration
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24. Endoplasmic reticulum stress in neurodegenerative diseases
- Prof. Claudio Soto
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25. Roles for Hsp40 molecular chaperones in protein misfolding disease
- Prof. Douglas M. Cyr
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26. Protein folding in vivo
- Prof. James Bardwell
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27. Protein degradation and defense against neurodegenerative disease 1
- Prof. Alfred Goldberg
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28. Protein degradation and defense against neurodegenerative disease 2
- Prof. Alfred Goldberg
Printable Handouts
Navigable Slide Index
- Introduction
- Protein folding in vitro
- Protein folding in vivo
- Interests
- Chaperones
- HdeA protects E. coli from stomach acid
- E. coli under non-stress conditions
- HdeA mechanism
- FRET to monitor HdeA conformation
- HdeA unfolding monitored by FRET
- HdeA adaptively binds substrates
- HdeA releases substrates very slowly
- Monitoring structural changes in HdeA
- Examine HdeA
- Examine substrate
- In vivo folding
- Ways to measure folding and stability
- To better understand in vivo folding
- A tripartite fusion system
- Quantitative in vivo stability readout
- Proteins inserted into beta-lactamase
- Selection for stabilized IM7 variants
- PenV-resistant IM7 mutants are stabilized
- Most proteins are only marginally stable
- Why are proteins so unstable?
- Stabilizing mutations map to binding interface
- Optimizing folding in vivo
- A dual selection system
- Effect instability of the inserted protein
- Fold or die!
- IM7 and Spy in the periplasm of EMS strains
- BaeS regulates Spy
- Spy overproduction is sufficient to stabilize IM7
- Spy inhibits MDH aggregation
- Spy suppresses protein aggregation
- Spy enhances protein refolding
- Spy and tannins
- Spy forms a thin cradle shaped dimer
- Substrate binding and environment changes
- Optimization of in vivo folding
- Acknowledgments
Topics Covered
- Protein folding in vivo
- Protein folding as facilitated by the acid inducible chaperone HdeA
- Optimization of folding in vivo illustrates a tradeoff between stability and function
- The Spy chaperone
Talk Citation
Bardwell, J. (2012, February 2). Protein folding in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 21, 2025, from https://doi.org/10.69645/BSRG3625.Export Citation (RIS)
Publication History
- Published on February 2, 2012
Financial Disclosures
- Prof. James Bardwell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.