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1. Introduction
2. Stochastic production of abnormal proteins
3. Protein quality control mechanisms (PQC)
4. PQC locations (1)
5. Protein aggregation diseases of the nucleus
6. Nuclear PQC mechanisms in yeast
7. Is SAN1 a nuclear PQC gene
8. Key features of a PQC degradation system (1)
9. Loss of SAN1 stabilizes mutant proteins
10. Mutant proteins specifically targeted by san1
11. San1 Is nuclear localized
12. How San1 functions
13. The San1 degradation pathway
14. San1's topology
15. San1 interacts with substrates via 2-hybrid
16. San1 ubiquitinates substrates in vitro
17. San1 is predicted to be disordered
18. San1 is intrinsically disordered by CD
19. What high disorder means
20. How San1 uses its flexible regions
21. Prediction of San1 binding sites
22. 2 models
23. San1 distinctly interacts with each substrate
24. Models of substrate binding
25. San1 recognition of misfolded substrates
26. Key features of a PQC degradation system (2)
27. San1 and hydrophobicity
28. Hydrophobicity is necessary and sufficient
29. San1-interacting & non-interacting peptides
30. Hydrophobicity as a San1 substrate determinant
31. Substrates with complex hydrophobicity profiles
32. Substrates with simple hydrophobicity profiles
33. Rad16* hydrophobic region
34. Ura3 temperature-sensitive point mutants
35. Exposed hydrophobicity in Ura3 (1)
36. Exposed hydrophobicity in Ura3 (2)
37. Consequences of exposed hydrophobicity
38. Many San1 substrates are toxic
39. Rad16* hydrophobicity important in San1 toxicity
40. Rad16* aggregates in the cell
41. Non aggregating Rad16* mutant
42. Important features of the San1 PQC
43. Other nuclear PQC degradation pathways
44. Potential nuclear PQCs - Yeast, Mammals
45. The nucleus as a final destination
46. PQC locations (2)
47. Cytoplasmic misfolded proteins (1)
48. Cytoplasmic misfolded proteins (2)
49. Cytoplasmic chaperones and nuclear trafficking
50. Open questions for nuclear PQC degradation
51. Acknowledgements
52. References

Topics Covered

• Ubiquitin-mediated degradation of misfolded proteins in the nucleus
• Protein aggregation in the nucleus
• Toxicity of protein aggregates

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on February 2, 2012

Financial Disclosures

• Dr. Richard G. Gardner has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.