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1. Introduction
2. It is important to explore
3. Knowledge of intracellular processes
4. Mechanisms of intracellular proteostasis
5. Coral spawn allusion
6. Knowledge of extracellular processes
7. Importance of extracellular proteostasis
8. Prominent amyloid diseases
9. Other protein aggregation diseases
10. Extracellular refolding
11. Selective extracellular proteolysis
12. Labelling and removal
13. Serendipity is a wonderful thing
14. Clusterin inhibits protein aggregation in stress (1)
15. Clusterin inhibits protein aggregation in stress (2)
16. Clusterin-client protein complexes
17. Identified extracellular chaperones
18. ECs in human plasma
19. Depletion of ECs from plasma
20. Clusterin KO mice
21. Circulating alpha2M and beta2m complexes
22. ECs role in protein aggregation and clearance
23. Structure of alpha2M
24. Protease trapping action of alpha2M
25. Chaperone/protease inhibitor function
26. Hypothetical scheme for in vivo roles of alpha2M
27. Current research foci
28. Radiolabelled clusterin-substrate complexes
29. Biodistribution of complexes in rats
30. Intracellular fate of complexes
31. Inducing shear stress in plasma
32. Fishing for client proteins
33. Endogenous clients for clusterin
34. A model for extracellular proteostasis
35. Motor neuron disease
36. Intracellular protein inclusions
37. Flow cytometry to purify inclusions
38. Collaborators
39. References

Topics Covered

• Extracellular proteostasis
• Mechanisms of intracellular proteostasis
• Disease relevance and potential mechanisms of extracellular proteostasis
• Extracellular chaperones, mechanism and abundance
• Alpha-2-macroglobulin
• Fate of extracellular chaperone-client protein complexes in vivo
• Model of extracellular proteostasis
• Clusterin can also enter the cytosol (Motor neurone disease)

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on February 2, 2012

Financial Disclosures

• Prof. Mark Wilson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.