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1. Introduction
2. Breakdown of presentation
3. Distribution of cytosine methylation in mammals
4. There is no single pattern of cytosine methylation
5. Cytosine methylation is inherently variable
6. CpG islands
7. CG ratio distributions by species
8. The paradox of CpG islands and DNMTs
9. Transposable elements
10. Distribution of cytosine methylation in humans
11. Shotgun bisulphite sequencing {MethylC-seq} (1)
12. Shotgun bisulphite sequencing {MethylC-seq} (2)
13. Shotgun bisulphite sequencing {MethylC-seq} (3)
14. Decreased methylation in promoter/CpG islands
15. Sites of modestly decreased methylation
16. Broad hypomethylated domains
17. Gene body methylation and gene expression (1)
18. Hypomethylation of enhancers
19. Gene body methylation and gene expression (2)
20. Non CG methylation
21. Non CG methylation biochemically plausible
22. Non-CG methylation may be inducible
23. Methylation distinctive at intron-exon boundaries
24. Influences on targeting of cytosine methylation
25. Innate sequence preferences of DNMTs
26. DNMT-DNA complex structure
27. CG dinucleotide periodicity in the human genome
28. Cell/tissue specific methylation pattern unsolved
29. Do chromatin modifications influence targeting?
30. Enzyme complexes potentially target the DNA
31. Enzyme complexes that target the DNA (1)
32. Enzyme complexes that target the DNA (2)
33. Transcription-targeted cytosine methylation
34. Are small RNAs influencing targeting?
35. Targeting of cytosine methylation - conclusions
36. DNA demethylation - three possible mechanisms
37. Methylcytosine mutation and repair
38. Protection against cytosine methylation (1)
39. Protection against cytosine methylation (2)
40. Protection against cytosine methylation (3)
41. Dynamics of cytosine methylation
42. DNA methylation is reorganized during development
43. Paternal demethylation in early embryogenesis
44. Elp3 is involved in paternal demethylation
45. Changes in cytosine methylation in disease
46. Rat IUGR study
47. Gene expression and methylation state correlate
48. Human disease studies - future challenges
49. Polymorphism may influence cytosine methylation
50. Conclusions and future directions
51. Acknowledgements

Topics Covered

• The distribution of cytosine methylation in mammals
• Influences on targeting of cytosine methylation
• Demethylation of DNA
• Protection against cytosine methylation
• Global and developmental dynamics of cytosine methylation
• Patterns of change of cytosine methylation in disease
• Influence of DNA sequence polymorphism on cytosine methylation variability
• Future directions

Links

Series:

• DNA Methylation

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on March 3, 2011

Financial Disclosures

• Prof. John Greally has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.