Hello and welcome to this Henry Stewart Talk
on "Techniques Utilized in Molecular Genetics".
My name is Cecil Lewis.
I'm an anthropologist at the University of Oklahoma,
and my research specializes in anthropological genetics.
I focused on questions related to the peopling of the world,
as well as the role of natural selection in the human genome.
I've also done some recent research on human microbiomes.
These are the collective genomes of a bacterial ecology
located on a human such as the microbiome located in saliva, or in feces.
There are wide range of techniques utilized in molecular genetics.
In this talk, I've grouped them under three general themes.
One is genetic material separation.
This includes techniques such as DNA isolation,
and mRNA isolation, library formation, and cell cultures.
Second is DNA amplification.
This is making multiple copies of
a particular genetic locus that you might be interested in.
This is done by methods such as PCR and molecular cloning.
Finally, I get at allele characterization.
Allele characterization is where we're actually trying to determine what is
the variation observed at the molecular genetic level?
Number of techniques used include gel electrophoresis,
probe hybridization, Real-Time PCR, and DNA sequencing.
But we'll get to these soon.