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Navigable Slide Index

1. Introduction
2. Outline
3. Comparative genome hybridisation
4. Conventional CGH
5. Metaphase spread hybridised in CGH experiment
6. CGH on microarrays
7. 1Mb resolution array
8. Array CGH
9. Array CGH - example
10. Conventional CGH vs. array CGH
11. CNVs are present in normal individuals
12. Assaying CNVs - BAC arrays
13. Genome tilepath array (1)
14. Genome tilepath array (2)
15. Survey of CNVs in human population
16. Survey of CNVs in human population - results
17. Assaying CNVs - long oligo G-W arrays
18. Whole-genome long oligo array
19. Long oligo array vs. BAC array
20. Resolution of the long oligo array
21. Assaying CNVs - fosmid arrays
22. Fosmid arrays
23. BAC vs. fosmid array
24. Redundant fosmid arrays
25. Analysis of chromosome 22 breakpoint (1)
26. Analysis of chromosome 22 breakpoint (2)
27. Assaying CNVs - small insert library arrays
28. Identification of chromosome specific markers
29. BAC vs. fosmid vs. small-insert clones array
30. Assaying CNVs - PCR amplicon arrays
31. PCR amplicon arrays
32. PCR amplicon arrays - example
33. Assaying CNVs - tiling long oligo arrays
34. Resolution of the 42M probe oligo array (1)
35. Resolution of the 42M probe oligo array (2)
36. CNV at 42M oligo-tiling resolution
37. Complexity of known CNVs on chr1
38. Perspectives
39. Summary
40. Acknowledgements

Topics Covered

• Array Comparative Hybridization (array-CGH) and measurement of copy number
• Development of array-CGH technology from convention CGH on metaphase chromosomes
• Initial use for identifying copy number changes in tumors
• Identification of normal copy number variation
• Increasing resolution of array-CGH reveals the scope, complexity and importance of normal copy number variation

Links

Series:

• Copy Number Variation

Categories:

• Genetics & Epigenetics
• Methods

Talk Citation

Carter, N. (2009, August 30). Array comparative genomic hybridization to characterize copy number variation in the human genome [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/UOEI4279.
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Publication History

• Published on August 30, 2009

Financial Disclosures

• Dr. Nigel Carter has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.