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0:00
Hello. My name is
Nancy Everds and I'm
a veterinary
clinical pathologist
at Amgen in Seattle Washington.
Today I'm going to talk
about the interpretation of
hematologic parameters
for toxicity studies.
0:14
Before we get very
far into this talk,
I want to go over what
the core hematology
parameters are.
These are generally included
in what we call the
complete blood count.
That includes red blood
cell mass parameters which
are red blood cell count
hemoglobin and hematocrit.
The red blood cell
indices which are
mean cell volume,
mean cell hemoglobin,
mean cell hemoglobin
concentration
and red cell distribution width.
The absolute
reticulocyte count which
is done on almost
every toxicity study.
The white blood cell count and
absolute differential white
blood cell counts and
two platelet parameters
usually the platelet count
and the mean platelet volume.
It's also important
to go ahead and
prepare a blood smear
even though you may not
need it to generate
the above parameters
because if you don't make
it at the time of analysis,
you can't go back
and make that smear.
It's really important to
always prepare a blood smear.
1:09
One of the most important
aspects of conducting
hematology tests on
toxicity studies
is the minimization
of variability.
It's really critical to
being able to generate
interpretable results.
Some of the potential sources of
variability include
physiologic effects,
procedural effects,
analytical effects,
artifacts either during
collection or analysis and
just basic study
design procedures
that impact the clinical
pathology result.
