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Printable Handouts
Navigable Slide Index
- Introduction
- Nervous systems evaluation
- Looking at the right place
- The brain is a heterogeneous tissue
- General toxicology examination of the brain
- Rules of thumb for sampling
- Sectioning interval for 60 sections
- Creating a sectioning scheme
- Trimming scheme
- The use of the trimming method
- Trimming scheme - dog brain
- Trimming scheme - rodent brain
- The spinal cord
- Looking at the right times
- Each compound has its own cell death peak
- Rat brain treated with MK801 (1)
- Rat brain treated with MK801 (2)
- The role of imaging in toxicology studies
- Looking in the right way
- Tissue changes are not created equal
- Perfusion fixation
- Neuronal necrosis stains
- Cupric silver stain
- Fluoro Jade B stain
- IBA-1 stain
- GFAP stain
- Luxol fast blue vs. classic silver stain
- Special procedures example
- Comparison of embedding and staining techniques
- Examples of high quality samples
- Longitudinal nerve sections (1)
- Longitudinal nerve sections (2)
- Electron micrograph
- Summary
- Thank you and acknowledgements
Topics Covered
- Brain is a heterogeneous tissue: you must look at many sections
- Different toxicants affect different areas
- Timing is important but most cell death occurs early (1 to 4 days)
- Appropriate fixation is crucial
- Appropriate staining methods are necessary to detect changes in various cell types (neurons, glial cells)
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Talk Citation
Butt, M. (2009, July 30). Evaluation of the adult nervous system in preclinical studies [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 24, 2024, from https://doi.org/10.69645/AJCG4690.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Mark Butt has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.