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1. Introduction
2. Cell cycle checkpoints for partitioning the genome
3. Budding yeast as a model system
4. Nuclei segregation in yeast (1)
5. Nuclei segregation in yeast (2)
6. Spindle position checkpoint
7. Evidence for a spindle position checkpoint (1)
8. Evidence for a spindle position checkpoint (2)
9. Before spindle moves into neck
10. After spindle moves into neck
11. Movie assay for checkpoint - normal
12. Movie assay for checkpoint - delayed
13. Movie assay for checkpoint - inappropriate
14. Movie assay for mitotic cell
15. GAP mutants show a complete loss of checkpoint
16. Lte1p - Putative GEF for Tem1
17. Inappropriate mitotic exit (1)
18. Inappropriate mitotic exit (2)
19. Putative MT sensor of neck
20. Screen for checkpoint mutations
21. Septins (1)
22. Septins (2)
23. Dual role of septins (1)
24. Dual role of septins (2)
25. Flow of information controlling mitotic exit (1)
26. Lte1 interacting genes and proteins
27. Bud6 mediates MT capture
28. Potential biochemical pathway
29. Null mutants have checkpoint defects
30. Flow of information controlling mitotic exit (2)
31. Defects are suppressed by loss of Lte1
32. Double mutants aren't worse than single mutants
33. Bud6 interaction with MT ends
34. Mutations that affect Bud6/MT interactions
35. Kip2 null mutant has a checkpoint phenotype
36. Overexpression analysis for pathway order
37. Mitotic exit with a MT present in the neck
38. Conclusions for Bud6
39. Inhibiting Lte1 is important in the cold
40. Cdc5 and Kin4 control mitotic exit
41. Future questions and directions

Topics Covered

• Cell cycle checkpoints for partitioning the genome
• Budding yeast as a model system for the study of genome distribution
• How nuclei are segregated in yeast
• Evidence for a spindle position checkpoint
• Model for checkpoint based on Tem1
• Movie assay for checkpoint
• Search for upstream inputs
• Screen for checkpoint mutants
• Septins dual role in checkpoint
• Inputs to Lte1
• Bud6 and microtubule capture
• Potential pathway for Bud6
• Bud6 and microtubule-binding proteins
• Overexpression analysis
• Inhibition of Lte1 in the cold

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology
• Microbiology

Talk Citation

Cooper, J. (2009, April 30). Spindle movement and checkpoint control during mitosis in yeast [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/WEKY7033.
Export Citation (RIS)

Publication History

• Published on April 30, 2009

Financial Disclosures

• Prof. John Cooper has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.