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- Chromosome Duplication
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1. Nucleosome assembly during DNA replication
- Dr. Alain Verreault
- Spindle Assembly and Chromosome Segregation
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2. Chromosome bi-orientation in yeast
- Prof. Mike Stark
- Prof. Tomo Tanaka
- The Cell Cycle in Development and Cancer
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3. Mouse models to investigate cell cycle and cancer
- Dr. Philipp Kaldis
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4. The Myc transcription factor network
- Prof. Robert N. Eisenman
- Archived Lectures *These may not cover the latest advances in the field
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5. Geometric regulation of kinetochore orientation
- Prof. Yoshinori Watanabe
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6. The spindle checkpoint
- Dr. Kevin Hardwick
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7. Cleavage furrow formation and ingression during animal cytokinesis
- Dr. Pier Paolo D'Avino
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8. Spindle movement and checkpoint control during mitosis in yeast
- Prof. John Cooper
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9. The G2/M transition
- Prof. Dr. René Medema
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11. Role and regulation of Cdk inhibitors in development and cancer
- Prof. Martine Roussel
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12. Recombination and the formation of chiasmata in meiosis
- Prof. Matthew Whitby
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13. Cell cycle: a complex network of signals regulating cell proliferation
- Prof. Antonio Giordano
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14. Drug discovery and target validation in the p53 pathway
- Prof. Sir David Lane
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15. START control in yeast
- Prof. Curt Wittenberg
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16. The pRB/E2F pathway
- Prof. Jacqueline Lees
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17. Cell cycle control by the ubiquitin system in mammals
- Prof. Michele Pagano
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18. Replication licensing
- Prof. Julian Blow
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19. Initiation of DNA replication
- Prof. Bruce Stillman
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20. Regulation of replication fork progression and stability
- Dr. Luis Aragón
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21. Sister chromatid cohesion: simple concept, complex reality
- Prof. Douglas Koshland
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22. Mitotic chromosome condensation
- Prof. Andrew Belmont
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23. Centrosome duplication and separation in animal cells
- Prof. Andrew Fry
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25. The DNA damage response
- Dr. Vincenzo Costanzo
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26. Bipolar spindle assembly
- Dr. Eric Karsenti
Printable Handouts
Navigable Slide Index
- Introduction
- Cell cycle checkpoints
- The metaphase to anaphase transition
- The spindle checkpoint
- What is the checkpoint for?
- Movie of mitosis and the spindle checkpoint
- What do cells monitor?
- Unattached kinetochores
- Lack of tension
- Checkpoint components
- Two budding yeast genetic screens
- Microcolony assay
- Checkpoint components - specific proteins
- Localisation of Mad proteins
- Checkpoint proteins recruited to kinetochores
- Role of kinetochores
- Global signalling
- FRAP analysis of checkpoint proteins turnover
- Identification of Cdc20 as checkpoint effector
- What is Cdc20?
- Structural insights of Mad1 and Mad2 (1)
- Structural insights of Mad1 and Mad2 (2)
- The presence of two distinct pools of Mad2
- Model: Mad1/Mad2 complex as a template
- Mad2 dimers
- The anaphase promoting complex (APC)
- How is anaphase onset inhibited?
- Mad2 and Mad3 bind mitotic APC
- Separase regulation by securin
- Cohesion in anaphase and metaphase
- Chromosomal Passenger Proteins (CPC)
- How to sense a lack of tension?
- Checkpoint role(s) of Aurora kinase (Ipl1)
- Checkpoint silencing
- p31comet-Mad2 "dimers"
- Disease relevance
- Future directions
- Acknowledgments
Topics Covered
- Cell cycle checkpoints
- The metaphase to anaphase transition
- The spindle checkpoint
- What do cells monitor?
- Unattached kinetochores
- Lack of tension
- Checkpoint components
- Budding yeast genetic screens
- Microcolony assay
- Localisation of Mad proteins
- Checkpoint proteins are all recruited kinetochores
- Role of kinetochores
- Global signaling
- Identification of Cdc20 as the checkpoint effector
- CdC20-APC
- FRAP
- Mad1/Mad2 complex as a template
- The anaphase promoting complex (APC)
- How is anaphase onset inhibited
- Mad2 and Mad3 bind mitotic APC
- Separase regulation by securin
- Error correction
- Chromosomal passenger proteins
- How to sense a lack of tension?
- Checkpoint role(s) of Aurora kinase (Ipl1)
- Checkpoint silencing
- Disease relevance
- Future directions
Talk Citation
Hardwick, K. (2009, April 30). The spindle checkpoint [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved July 5, 2025, from https://doi.org/10.69645/AGXJ1940.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Kevin Hardwick has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.