• Co-ordination of G1 Progression
• 37 min
  1. START control in yeast

  • Prof. Curt Wittenberg
• 33 min
  2. The pRB/E2F pathway

  • Prof. Jacqueline Lees
• 35 min
  3. Cell cycle control by the ubiquitin system in mammals

  • Prof. Michele Pagano
• Chromosome Duplication
• 47 min
  4. Replication licensing

  • Prof. Julian Blow
• 51 min
  5. Initiation of DNA replication

  • Prof. Bruce Stillman
• 48 min
  6. Regulation of replication fork progression and stability

  • Dr. Luis Aragón
• 35 min
  7. Nucleosome assembly during DNA replication

  • Dr. Alain Verreault
• Preparing for Mitosis
• 27 min
  8. Sister chromatid cohesion: simple concept, complex reality

  • Prof. Douglas Koshland
• 50 min
  9. Mitotic chromosome condensation

  • Prof. Andrew Belmont
• 33 min
  10. Centrosome duplication and separation in animal cells

  • Prof. Andrew Fry
• Spindle Assembly and Chromosome Segregation
• 30 min
  11. Bipolar spindle assembly

  • Dr. Eric Karsenti
• 54 min
  12. Chromosome biorientation in yeast

  • Prof. Mike Stark
• 43 min
  13. Regulation of mammalian cell division by the chromosomal passenger complex

  • Dr. Susanne Lens
• Mitotic Exit and Cytokinesis
• 39 min
  14. Cleavage furrow formation and ingression during animal cytokinesis

  • Dr. Pier Paolo D'Avino
• Checkpoints Governing Cell Cycle Progression
• 34 min
  15. The DNA damage response

  • Dr. Vincenzo Costanzo
• 54 min
  16. The spindle checkpoint

  • Dr. Kevin Hardwick
• 27 min
  17. Spindle movement and checkpoint control during mitosis in yeast

  • Prof. John Cooper
• 43 min
  18. The G2/M transition

  • Prof. Dr. René Medema
• The Cell Cycle in Development and Cancer
• 62 min
  19. Mouse models to investigate cell cycle and cancer

  • Dr. Philipp Kaldis
• 18 min
  20. Cell cycle: a complex network of signals regulating cell proliferation

  • Prof. Antonio Giordano
• 40 min
  21. Drug discovery and target validation in the p53 pathway

  • Prof. Sir David Lane
• 30 min
  22. Role and regulation of Cdk inhibitors in development and cancer

  • Prof. Martine Roussel
• 48 min
  23. The kinetochore as a target for the development of mitosis specific anti-cancer drugs

  • Dr. Tim Yen
• 47 min
  24. The Myc transcription factor network

  • Prof. Robert N. Eisenman
• Meiosis: A Specialized Cell Cycle
• 58 min
  25. Recombination and the formation of chiasmata in meiosis

  • Prof. Matthew Whitby
• Archived Lectures *These may not cover the latest advances in the field
• 24 min
  26. Geometric regulation of kinetochore orientation

  • Prof. Yoshinori Watanabe

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Cell cycle checkpoints
3. The metaphase to anaphase transition
4. The spindle checkpoint
5. What is the checkpoint for?
6. Movie of mitosis and the spindle checkpoint
7. What do cells monitor?
8. Unattached kinetochores
9. Lack of tension
10. Checkpoint components
11. Two budding yeast genetic screens
12. Microcolony assay
13. Checkpoint components - specific proteins
14. Localisation of Mad proteins
15. Checkpoint proteins recruited to kinetochores
16. Role of kinetochores
17. Global signalling
18. FRAP analysis of checkpoint proteins turnover
19. Identification of Cdc20 as checkpoint effector
20. What is Cdc20?
21. Structural insights of Mad1 and Mad2 (1)
22. Structural insights of Mad1 and Mad2 (2)
23. The presence of two distinct pools of Mad2
24. Model: Mad1/Mad2 complex as a template
25. Mad2 dimers
26. The anaphase promoting complex (APC)
27. How is anaphase onset inhibited?
28. Mad2 and Mad3 bind mitotic APC
29. Separase regulation by securin
30. Cohesion in anaphase and metaphase
31. Chromosomal Passenger Proteins (CPC)
32. How to sense a lack of tension?
33. Checkpoint role(s) of Aurora kinase (Ipl1)
34. Checkpoint silencing
35. p31comet-Mad2 "dimers"
36. Disease relevance
37. Future directions
38. Acknowledgments

Topics Covered

• Cell cycle checkpoints
• The metaphase to anaphase transition
• The spindle checkpoint
• What do cells monitor?
• Unattached kinetochores
• Lack of tension
• Checkpoint components
• Budding yeast genetic screens
• Microcolony assay
• Localisation of Mad proteins
• Checkpoint proteins are all recruited kinetochores
• Role of kinetochores
• Global signaling
• Identification of Cdc20 as the checkpoint effector
• CdC20-APC
• FRAP
• Mad1/Mad2 complex as a template
• The anaphase promoting complex (APC)
• How is anaphase onset inhibited
• Mad2 and Mad3 bind mitotic APC
• Separase regulation by securin
• Error correction
• Chromosomal passenger proteins
• How to sense a lack of tension?
• Checkpoint role(s) of Aurora kinase (Ipl1)
• Checkpoint silencing
• Disease relevance
• Future directions

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology

Therapeutic Areas:

• Oncology

Talk Citation

Publication History

• Published on April 30, 2009

Financial Disclosures

• Dr. Kevin Hardwick has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.