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1. Introduction
2. Cell cycle checkpoints
3. The metaphase to anaphase transition
4. The spindle checkpoint
5. What is the checkpoint for?
6. Movie of mitosis and the spindle checkpoint
7. What do cells monitor?
8. Unattached kinetochores
9. Lack of tension
10. Checkpoint components
11. Two budding yeast genetic screens
12. Microcolony assay
13. Checkpoint components - specific proteins
14. Localisation of Mad proteins
15. Checkpoint proteins recruited to kinetochores
16. Role of kinetochores
17. Global signalling
18. FRAP analysis of checkpoint proteins turnover
19. Identification of Cdc20 as checkpoint effector
20. What is Cdc20?
21. Structural insights of Mad1 and Mad2 (1)
22. Structural insights of Mad1 and Mad2 (2)
23. The presence of two distinct pools of Mad2
24. Model: Mad1/Mad2 complex as a template
25. Mad2 dimers
26. The anaphase promoting complex (APC)
27. How is anaphase onset inhibited?
28. Mad2 and Mad3 bind mitotic APC
29. Separase regulation by securin
30. Cohesion in anaphase and metaphase
31. Chromosomal Passenger Proteins (CPC)
32. How to sense a lack of tension?
33. Checkpoint role(s) of Aurora kinase (Ipl1)
34. Checkpoint silencing
35. p31comet-Mad2 "dimers"
36. Disease relevance
37. Future directions
38. Acknowledgments

Topics Covered

• Cell cycle checkpoints
• The metaphase to anaphase transition
• The spindle checkpoint
• What do cells monitor?
• Unattached kinetochores
• Lack of tension
• Checkpoint components
• Budding yeast genetic screens
• Microcolony assay
• Localisation of Mad proteins
• Checkpoint proteins are all recruited kinetochores
• Role of kinetochores
• Global signaling
• Identification of Cdc20 as the checkpoint effector
• CdC20-APC
• FRAP
• Mad1/Mad2 complex as a template
• The anaphase promoting complex (APC)
• How is anaphase onset inhibited
• Mad2 and Mad3 bind mitotic APC
• Separase regulation by securin
• Error correction
• Chromosomal passenger proteins
• How to sense a lack of tension?
• Checkpoint role(s) of Aurora kinase (Ipl1)
• Checkpoint silencing
• Disease relevance
• Future directions

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on April 30, 2009

Financial Disclosures

• Dr. Kevin Hardwick has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.