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Printable Handouts
Navigable Slide Index
- Introduction
- Cell cycle checkpoints
- The metaphase to anaphase transition
- The spindle checkpoint
- What is the checkpoint for?
- Movie of mitosis and the spindle checkpoint
- What do cells monitor?
- Unattached kinetochores
- Lack of tension
- Checkpoint components
- Two budding yeast genetic screens
- Microcolony assay
- Checkpoint components - specific proteins
- Localisation of Mad proteins
- Checkpoint proteins recruited to kinetochores
- Role of kinetochores
- Global signalling
- FRAP analysis of checkpoint proteins turnover
- Identification of Cdc20 as checkpoint effector
- What is Cdc20?
- Structural insights of Mad1 and Mad2 (1)
- Structural insights of Mad1 and Mad2 (2)
- The presence of two distinct pools of Mad2
- Model: Mad1/Mad2 complex as a template
- Mad2 dimers
- The anaphase promoting complex (APC)
- How is anaphase onset inhibited?
- Mad2 and Mad3 bind mitotic APC
- Separase regulation by securin
- Cohesion in anaphase and metaphase
- Chromosomal Passenger Proteins (CPC)
- How to sense a lack of tension?
- Checkpoint role(s) of Aurora kinase (Ipl1)
- Checkpoint silencing
- p31comet-Mad2 "dimers"
- Disease relevance
- Future directions
- Acknowledgments
Topics Covered
- Cell cycle checkpoints
- The metaphase to anaphase transition
- The spindle checkpoint
- What do cells monitor?
- Unattached kinetochores
- Lack of tension
- Checkpoint components
- Budding yeast genetic screens
- Microcolony assay
- Localisation of Mad proteins
- Checkpoint proteins are all recruited kinetochores
- Role of kinetochores
- Global signaling
- Identification of Cdc20 as the checkpoint effector
- CdC20-APC
- FRAP
- Mad1/Mad2 complex as a template
- The anaphase promoting complex (APC)
- How is anaphase onset inhibited
- Mad2 and Mad3 bind mitotic APC
- Separase regulation by securin
- Error correction
- Chromosomal passenger proteins
- How to sense a lack of tension?
- Checkpoint role(s) of Aurora kinase (Ipl1)
- Checkpoint silencing
- Disease relevance
- Future directions
Talk Citation
Hardwick, K. (2009, April 30). The spindle checkpoint [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/AGXJ1940.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Kevin Hardwick has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.