Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Cell cycle control of DNA replication
3. Yeast as a model eukaryote
4. Control of S phase entry in yeast
5. Characterization of origins of DNA replication
6. Modular structure of S. cerevisiae origins
7. The budding yeast origin recognition complex
8. Structure of the ORC-Cdc6 complex
9. Evolutionary comparison of ORC AAA+ subunits
10. DnaA and DnaC recognize OriC and load DnaB
11. Formation of the pre-RC
12. Association of initiation proteins with chromatin
13. ATP dependent assembly of the pre-RC
14. Cell cycle regulation of the pre-RC
15. Chromosome cycle and establishment of pre-RCs
16. Dynamics of ORC in human cells
17. Commitment to S phase and initiation
18. Cdc45p is associated with chromatin
19. Cdc45p ChIP during S phase
20. CDK and DDK-dependent assembly of pre-IC
21. By-pass of CDK for pre-IC assembly
22. Temporal regulation of DNA replication
23. Dynamics of pre-IC in human cells
24. Dynamics of ORC and Cdc6
25. Orc2 and the cell cycle
26. Initiation of DNA synthesis - the fork
27. SV40: a simple replicon
28. DNA replication-dependent chromatin assembly
29. Initiation of virus DNA replication
30. The "primasome" loading
31. E1 helicase activity on single strand DNA (1)
32. E1 helicase activity on single strand DNA (2)
33. PCNA as a DNA replication protein
34. Temporal organization of DNA replication
35. PCNA, MCM3 during cell division cycle
36. Conserved structure of polymerase clamps
37. Sequential events at the SV40 DNA replication fork
38. SV40 DNA replication fork
39. Eukaryotic cell DNA replication fork
40. Challenges for the future

Topics Covered

• Cell cycle control of DNA replication
• Yeast as a model eukaryote
• Control of S-phase entry in yeast
• Characterization of origins of DNA replication
• Modular structure of S. cerevisiae origins
• The budding yeast origin recognition complex (ORC)
• Structure of the ORC-Cdc6 complex
• Evolutionary comparison of ORC AAA+ subunits
• Proteins required for formation of the pre-Replication Complex (pre-RC)
• Cell cycle dependent association of yeast initiation proteins with chromatin
• ATP-dependent assembly of a pre-RC
• Cell cycle regulation of the pre-RC
• The chromosome cycle and establishment of pre-RCs
• Dynamic assembly and stability of ORC in human cells
• Commitment to S-phase and initiation
• Cdc45p is associated with chromatin in a cell cycle-dependent manner
• Temporal regulation of DNA replication
• Dynamics of pre-RC proteins in human cells differ from pre-RC proteins in yeast
• Dynamics of human cell ORC and Cdc6 during the cell division cycle
• Orc2 and the cell cycle
• Initiation of DNA synthesis: the fork
• SV40: a simple replicon
• DNA replication-dependent chromatin assembly
• Initiation of virus DNA replication
• Initiation of DNA synthesis
• E1 helicase activity on single stranded DNA
• Identification of PCNA as a DNA replication protein
• Temporal organization of DNA replication in human cell nuclei
• Conserved structure (not sequence) of polymerase clamps
• Sequential events at the SV40 DNA replication fork
• Proteins at the SV40 DNA replication fork
• Proteins at the eukaryotic cell DNA replication fork
• Challenges for the future

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on May 5, 2009

Financial Disclosures

• Prof. Bruce Stillman has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.