• The Notion of Epigenetics
• 43 min
  1. Cytoplasmic epigenetics: inheritance by cytoplasmic continuity

  • Prof. Philippe Silar
  • Dr. Fabienne Malagnac
• Epigenetics: Paradigms
• 38 min
  2. The molecular mechanism of X chromosome inactivation

  • Prof. Neil Brockdorff
• 29 min
  3. Genomic imprinting: history and embryology

  • Prof. Davor Solter
• 48 min
  4. X chromosome inactivation in human cells

  • Prof. Barbara Migeon
• 41 min
  5. RNAi and heterochromatin in plants and fission yeast

  • Prof. Robert Martienssen
• Epigenetics: Mechanisms
• 23 min
  6. Polycomb epigenetic mechanisms: role of PcG complexes

  • Prof. Vincenzo Pirrotta
• 29 min
  7. Polycomb epigenetic mechanisms: methylation of DNA

  • Prof. Vincenzo Pirrotta
• 56 min
  8. Histone modifications and prospects for an epigenetic code

  • Prof. Bryan Turner
• 68 min
  9. Epigenetic control by histone methylation

  • Prof. Thomas Jenuwein
• 38 min
  10. Histone dynamics, heritability and variants

  • Dr. Genevieve Almouzni
• 51 min
  11. Gene silencing in budding yeast

  • Prof. Susan Gasser
• Epigenetics: Heritability and Reversibility
• 51 min
  12. Nuclear cloning, stem cells and epigenetic reprogramming

  • Prof. Rudolf Jaenisch
• 64 min
  13. Stem cell memory

  • Prof. James Sherley
• Archived Lectures *These may not cover the latest advances in the field
• 24 min
  14. Epigenetics: a historical overview

  • Dr. Robin Holliday
• 43 min
  15. DNA methylation

  • Prof. Adrian Bird
• 45 min
  16. DNA methylation and genome defense in Neurospora crassa

  • Prof. Eric Selker
• 34 min
  17. A historical perspective on ideas on X-chromosome inactivation

  • Dr. Mary Lyon
• 40 min
  18. Evolution of mammal epigenetic control systems

  • Prof. Jenny Graves
• 60 min
  19. Genomic imprinting and its regulation

  • Dr. Anne Ferguson-Smith
• 40 min
  20. Nuclear organization and gene expression

  • Prof. David Spector
• 39 min
  21. Germ cells

  • Prof. Azim Surani
• 41 min
  22. Epigenetic regulation of phenotype

  • Prof. Emma Whitelaw
• 68 min
  23. Epigenetically mediated gene silencing: a companion to genetic alterations in driving tumorigenesis

  • Prof. Stephen Baylin
• 36 min
  24. Cytoplasmic epigenetics: proteins acting as genes

  • Prof. Reed Wickner

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Gene expression induced by extracellular ligand
3. Nuclear organization
4. Chromosomes arrangement in interphase nucleus
5. Visualizing gene expression in living cells
6. Characterization of the U2OS stable cell line
7. The locus is stably integrated in chr. 1p36
8. 2.5 h post-transfection (-) Dox
9. 2.5 h post-transfection + 2.5 h after Dox addition
10. Evidence that RNA is present at transcription site
11. Changes in chromatin structure after activation (1)
12. Changes in chromatin structure after activation (2)
13. 3-D organization of gene expres. 2 h after initiation
14. Nuclear mRNP dynamics consistent with diffusion
15. mRNP particle tracking
16. Tet-ON-VP16-YFP at the locus after Dox addition
17. Tet-ON-VP16-YFP recruited to the locus over time
18. RNA pol. II large subunit recruited to active locus
19. SF2/ASF recruited to active locus
20. CstF-64 recruited to active locus
21. Tri-methyl. histone H3 as a marker for HP1 binding
22. HP1 alpha/beta/gamma enriched at inactive locus
23. Tri-methyl. histone H3 enriched at inactive locus
24. Suv39h1/G9a-L HMTases localize at inactive locus
25. HP1 alpha clears from locus upon transcription (1)
26. HP1 alpha clears from locus upon transcription (2)
27. HP1 alpha clears from locus upon transcription (3)
28. Histone H3 and the H3.3 variant
29. Histone H3.3 enriched at the active locus (1)
30. Histone H3.3 enriched at the active locus (2)
31. Summary (1)
32. Summary (2)
33. Acknowledgements

Topics Covered

• Current understanding of the organization of the mammalian cell nucleus and how this organization impacts on gene expression
• in-vivo studies of gene expression as a result of the concerted processes of transcription, mRNA processing, messenger ribonucleoprotein particle (mRNP) export and translation
• studies of spatial and temporal aspects of critical cellular processes within the context of the living cell
• development of a cell system that allows the visualization of a transcription site and its mRNA and protein products directly in living cells
• evaluation of spatial and temporal changes in chromatin structure, mRNA synthesis and factor association/disassociation during the transition from a transcriptionally inactive to an active state
• understanding of the in-vivo dynamics of gene expression will provide the basis from which to elucidate spatial and/or temporal alterations that occur in cells associated with various disease states

Links

Series:

• Epigenetics
• From DNA to Proteins

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. David Spector has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.