Registration for a live webinar on 'Innovative Vaccines and Viral Pathogenesis: Insights from Recent Monkeypox Research' is now open.
See webinar detailsWe noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- Interpretation of gene expression data
- Microarray data analysis
- The central dogma of molecular biology
- The potential transcriptome space is n-dimensional
- Coordinate gene expression (1)
- Coordinate gene expression (2)
- The actual transcriptome space
- Evolution of current microarray technology
- Northern blots
- Dot blots: single gene, many tissues
- Dot blots: many genes, single tissue
- Microarrays: radioactivity on nylon
- What's measured by conventional microarrays?
- Combined analysis of microarray with NRO array
- The ratio between total RNA and NRO
- T cell activation model (1)
- T cell activation model (2)
- T cell activation model (3)
- mRNA stabilization and de-stabilization
- Gene regulation
- NRO gene expression is captured quickly
- Confiriming post transcriptional regulation (1)
- Confiriming post transcriptional regulation (2)
- NRO assay using biotin labeling
- Are we getting full length genes?
- NRO RNA microarray vs. RT-PCR
- Optimization by a number of parameters
- Whole cell mRNA vs. nascent gene transcription
- Over expressed myc in B cells
- 48 hours with and without myc
- Total RNA and NRO of cRNA
- Comparing affimetrix and illumina
- Regulation by microRNA
- Effect of a glucorticosteroid on epithelial cell line
- NRO RNA full genome analysis
- Acknowledgements
Topics Covered
- Conceptual frameworks for the interpretation of gene expression data
- Microarray data analysis
- The potential transcriptome space is n-dimensional
- Co-ordinate gene expression
- Evolution of current microarray technology
- Northern blots
- Radioactivity on nylon
- Combined analysis of conventional microarray with NRO array
- Jurkat T cell activation
- Global comparison of whole cell mRNA vs. nascent gene transcription
Talk Citation
Cheadle, C. (2009, March 30). Simultaneous microarray measurement of nuclear and whole cell changes in gene expression reveals overlapping but distinct patterns of transcriptional regulation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 7, 2024, from https://doi.org/10.69645/DDMI1555.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Chris Cheadle has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Simultaneous microarray measurement of nuclear and whole cell changes in gene expression reveals overlapping but distinct patterns of transcriptional regulation
A selection of talks on Cell Biology
Hide