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1. DNA methylation and genome defense: N. crassa
2. X-inactivation: process involving DNA methylation
3. N. crassa: vegetative/sexual phases of life cycle
4. N. crassa: 5S rRNA gene distribution in genome
5. The zeta-eta region
6. DNA methylation in N. crassa
7. Initial questions
8. De-novo methylation of zeta-eta region
9. Repeat-induced point mutation (RIP)
10. RIP produces excess TpA's
11. RIP and DNA methylation: questions
12. Isolation of methylated DNA with MBD-column
13. Most products of RIP are relics of transposons
14. Most predicted relics of RIP are methylated
15. RIP and DNA methylation: answers
16. RIP is an efficient genome defense mechanism
17. N. crassa: multigene families and gene pairs
18. RIP'd sequences and methylated sequences
19. Mutations and methylation of amRIP alleles
20. Repeat-induced point mutation (RIP)
21. Potential DNA MTases of N. crassa
22. Possible mechanisms of RIP
23. How RIP'd sequences trigger DNA methylation?
24. Dissection of methylation signal generated by RIP
25. Induction of methylation by A:T 25-mers
26. G:C inhibit methylation by A:T rich sequences
27. TpA and ApT are not needed to trigger methylation
28. Distamycin inhibits DNA methylation in N. crassa
29. Genetic approach to elucidate DNA methylation
30. Identification of dim-5
31. DIM-5 trimethylates Lys9 in H3 peptides
32. Potential role of histone H3 in DNA methylation
33. Histone H3 Lys9 substitutions
34. DIM-5 is responsible for trimethylation of H3 Lys9
35. Does DIM-5 "read" (or is it just "guided")?
36. H3 peptide lies in surface groove of DIM-5
37. Role of histone deacetylation in DNA methylation
38. Sites of modification in histone H3 N-terminal tail
39. Putative N. crassa HP1
40. N. crassa HP1 is required for DNA methylation
41. Localization of HP1-GFP
42. DNA methylation control in N. crassa: basic model
43. DNA methylation/heterochromatin in RNAi mutants
44. Key points
45. References

Topics Covered

• Control of DNA methylation
• repeat-induced point (RIP) mutation
• relationship of chromatin modifications and DNA methylation
• mutants that eliminate DNA methylation
• roles of histone H3 methyltransferase (DIM-5) and heterochromatin protein 1 (HP1) in DNA methylation
• indications of involvement of protein acetylation, methylation and phosphorylation in mechanism of DNA methylation
• propagation of DNA methylation and the associated silenced chromatin state

Links

Series:

• Epigenetics

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Selker, E. (2007, October 1). DNA methylation and genome defense in Neurospora crassa [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/YXHU3176.
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Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Eric Selker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.