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Printable Handouts
Navigable Slide Index
- Introduction
- Peptide synthesis
- Peptide theory of protein structure
- Chemical structure of peptides
- (Chemical) peptide synthesis
- Epochs of chemical peptide synthesis
- Classical solution synthesis
- Protein-derived peptides
- Solid phase peptide synthesis
- Chemistry of SPPS
- SPPS - reaction vessel
- SPPS case study: cecropin A
- SPPS case study: human beta-endorphin
- Automated solid phase peptide synthesis
- Impact of solid phase peptide synthesis
- Fundamentals of SPPS
- Synthesis takes place within the beads
- 'Super-elastic' swelling of peptide-resin beads
- Narrow-line NMR spectra of resin-bound peptides
- Solubilizing effect of attachment to the resin
- Functionalization of the resin support
- Boc chemistry SPPS - protecting group scheme
- Boc chemistry SPPS - cleavable linkers
- Boc chemistry SPPS - protecting groups
- Boc chemistry SPPS - chain extension
- Boc chemistry SPPS - side reactions
- Boc chemistry SPPS - deprotection and cleavage
- Boc chemistry SPPS - HF apparatus
- Boc chemistry SPPS - HF side reactions
- Fmoc chemistry SPPS - protecting group scheme
- Fundamentals of Fmoc chemistry SPPS
- Fmoc chemistry SPPS - cleavable linkers
- Chain extension and deprotection/cleavage
- 'Difficult' peptide sequences in SPPS
- The 'diffculty' is a property of the peptide chain
- Examples of 'difficult' peptide sequences for SPPS
- Effect of disrupting peptide aggregation
- An optimized solid phase peptide synthesis
- Boc SPPS case study: HIV-1 protease
- Boc SPPS case study: DGK(1-25)-thioester
- Optimized Fmoc SPPS
- Fmoc SPPS case study: transmembrane peptide
- Fmoc SPPS case study: FDD(50-95)-amide
- Impact of modern analytical methods on SPPS
- Preparation of large numbers of natural peptides
- Extensions and applications of SPPS
- Scope and limitations of SPPS
- Byproducts accumulate as chain length increases
- Synthesis of HIV-1 protease
- Capability of routine chemical protein synthesis
- Chemical protein synthesis
- Synthesis of transmembrane peptide-thioesters
- Impact of chemical ligation
- Emil Fischer (1852-1919)
- Total synthesis of human lysozyme
- Impact of SPPS
- Bruce Merrifield (1921-2006)
Topics Covered
- Chemical structure of peptides
- Chemical peptide synthesis
- Classical solution synthesis
- Solid-phase peptide synthesis
- Chemistry of SPPS
- Automated solid-phase peptide synthesis
- Impact of SPPS
- Fundamentals of SPPS
- Synthesis takes place within the beads
- 'Super-elastic' swelling of peptide-resin beads
- Boc chemistry SPPS
- Fmoc chemistry SPPS
- 'Difficult' peptide sequences in SPPS
- Optimized solid-phase peptide synthesis
- Impact on SPPS of modern analytical methods
- Extensions of SPPS & applications of the solid-phase principle
- Scope and limitations of SPPS
- Impact of chemical ligation
- Total synthesis of human lysozyme
Talk Citation
Kent, S. (2008, August 14). Solid phase peptide synthesis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 20, 2024, from https://doi.org/10.69645/WHCY3062.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Stephen Kent has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.