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1. Introduction
2. Gastrulation in Drosophila
3. Studies in humans vs. fly
4. Embryonic development: gastrulation is key
5. Transcriptional regulation
6. Dorsal transcription factor morphogen gradient
7. Genes regulated by Dorsal "pattern" the embryo
8. Dorsal protein levels control cell fate specification
9. Mutants containing 3 distinct levels of Dorsal
10. Dorsal patterns the dorsoventral axis of the embryo
11. Gene expression patterns in mutant backgrounds
12. Microarray and whole-genome expression analysis
13. Using microarrays to predict gene expression
14. 16 Dorsal target genes
15. Identify cis-regulatory sequences
16. Combined interactions among transcription factors
17. Classes of Dorsal threshold output enhancers
18. ChIP-chip analysis
19. ChIP-chip: antibodies to Dorsal, Twist, and Snail
20. Code for expression along the dorsoventral axis
21. The network controlling patterning and gastrulation
22. Dorsal-regulated genes
23. thisbe: a gene identified using microarrays (1)
24. thisbe: a gene identified using microarrays (2)
25. Gastrulation movements along the DV axis
26. Fibroblast growth factors
27. Fibroblast growth factor (FGF) signaling
28. FGF-ligands and receptors
29. ths and pyr are dynamically expressed
30. Improper mesoderm migration in mutant embryos
31. ths and pyr are required for heart cell specification
32. In mutant embryos heart is not properly formed
33. Functions for activation of FGF receptor Heartless
34. pyramus and thisbe expression domains
35. Fgf ligands have different activity levels
36. Why doesn't the mesoderm migrate properly?
37. Mesoderm migration - live
38. Movement of mesoderm cells
39. Individual cells tracks
40. Phenotype of pyr and ths double mutants
41. Illustration of migrating mesoderm cells
42. Summary
43. Acknowledgments

Topics Covered

• Gastrulation
• Dorsoventral patterning in the early embryo
• Activation of signaling pathways
• Control of cell movements and differentiation events
• Mesoderm cell spreading and heart cell specification
• FGF signaling during development

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Cell Biology
• Methods
• Reproduction & Development

Talk Citation

Stathopoulos, A. (2008, September 29). Using gene expression information to provide insights into patterning and differentiation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/MHDU5986.
Export Citation (RIS)

Publication History

• Published on September 29, 2008
• Archived on June 7, 2018

Financial Disclosures

• Prof. Angelike Stathopoulos has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.