• Introduction
• 63 min
  1. Drosophila genetics - the first 25 years

  • Prof. Dan Lindsley
• Establishment of the Primary Body Axes
• 28 min
  2. Homeotic genes in Drosophila's bithorax complex - The legacy of Ed Lewis

  • Prof. Francois Karch
• 30 min
  3. Limb development: how Drosophila genetics can be used to understand pattern formation

  • Prof. Konrad Basler
• Cell Type Specification and Organ Systems
• 63 min
  4. From germ cell specification to gonad formation

  • Prof. Ruth Lehmann
• 37 min
  5. Drosophila stem cells

  • Prof. Michael Buszczak
• 46 min
  6. Legacy of drosophila genetics: female germline stem cells

  • Prof. Michael Buszczak
• 34 min
  7. Intestinal stem cell-mediated repair in Drosophila 1

  • Prof. Tony Ip
• 30 min
  8. Intestinal stem cell-mediated repair in Drosophila 2

  • Prof. Tony Ip
• 43 min
  9. The miracle of morphogenesis, cell adhesion, polarity and cytoskeletal regulation

  • Prof. Mark Peifer
• 34 min
  10. Axon guidance in Drosophila

  • Prof. John Thomas
• 35 min
  11. Development and physiology of the heart

  • Prof. Rolf Bodmer
• 37 min
  12. Identification of host defenses in the Drosophila gut using genome-scale RNAi

  • Prof. Dominique Ferrandon
• Genome Organization and Function
• 53 min
  13. The genetic analysis of meiosis in Drosophila melanogaster females

  • Prof. R. Scott Hawley
• 46 min
  14. Genome scale analysis of cellular processes using RNAi

  • Dr. Amy Kiger
• 36 min
  15. Dorsal-ventral patterning of the Drosophila embryo

  • Prof. Mike Levine
• 34 min
  16. Bone morphogenetic protein (BMP) signaling in drosophila: from germline to early embryogenesis

  • Dr. Helena Araujo
• 27 min
  17. Genome-wide pooled CRISPR screen in arthropod cells

  • Prof. Norbert Perrimon
• Behavior
• 40 min
  18. The development of the peripheral nervous system in the fruitfly Drosophila

  • Prof. Hugo Bellen
• 54 min
  19. Genetics of chemosensory transduction: taste and smell

  • Dr. Leslie Vosshall
• 72 min
  20. Cracking the case of circadian rhythms by Drosophila genetics

  • Prof. Jeffrey C. Hall
• 46 min
  21. Sleep in Drosophila

  • Dr. Ralph Greenspan
• 91 min
  22. Drosophila's contribution to the genetics of learning and memory

  • Prof. Tim Tully
• 34 min
  23. Drosophila as a model for drug addiction

  • Prof. Ulrike Heberlein
• Mechanism of Human Disease
• 65 min
  24. Cross-genomic analysis of human disease genes

  • Prof. Ethan Bier
• 56 min
  25. Human neurodegenerative disease: insights from Drosophila genetics

  • Prof. Nancy Bonini
• 21 min
  26. Metastasis of Drosophila tumors

  • Prof. Allen Shearn
• 
  27. Rac-enhanced CAR immunotherapy: RaceCAR

  • Prof. Denise Montell
• 34 min
  28. Control of tissue growth: elucidation of the Hippo signaling pathway

  • Dr. Duojia Pan
• Evolution of Adaptive Novelties
• 56 min
  29. The evolution of morphological novelty

  • Prof. Nipam Patel
• 36 min
  30. The genetic architecture of complex traits: lessons from Drosophila

  • Prof. Trudy Mackay
• Archived Lectures *These may not cover the latest advances in the field
• 25 min
  31. Using gene expression information to provide insights into patterning and differentiation

  • Prof. Angelike Stathopoulos
• 49 min
  32. Regulation of gastrulation in Drosophila

  • Prof. Dr. Maria Leptin
• 31 min
  33. microRNA function in stem cells

  • Prof. Hannele Ruohola-Baker

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Stem cells
3. Bristlecone pine
4. Control of stem cell division
5. microRNA and stem cells in model organisms
6. microRNA function in stem cells
7. Characteristics of microRNAs
8. Mutating Dicer and Drosha in Drosophila GSCs
9. Dcr-1 mutants
10. Germ-line stem cells location
11. GSC maintenance and self renewal
12. Notch pathway activation induces extra niche cells
13. GSC maintenance: niche to GSC signaling
14. Drosophila Dicer is required for GSC maintenance
15. Dicer and Mad are required for GSC maintenance
16. Analysis of microRNAs expression in GSC
17. miR-7, miR-8 and bantam are expressed in GSCs
18. Which microRNA is required in GSC?
19. Bantam is required for GSC self-renewal
20. Signaling pathways in GSC self-renewal: summary
21. microRNA control of stem cell division
22. Dicer and Mad in Drosophila larval/pupal clones
23. Drosophila oogenesis
24. Drosophila oogenesis in Dcr-1 mutants
25. Dcr1-mutant stem cells stop dividing!
26. Dcr1-mutant stem cells halt in G1/S transition
27. Is Dacapo a direct target of microRNAs?
28. Analysis of Dacapo regulation
29. Dacapo promoter is not responsive to Dicer
30. Do microRNAs directly regulate Dap 3'UTR?
31. Dap 3'UTR is responsive to miRNAs in GSCs
32. Dap 3'UTR is responsive to miR-7 and -309
33. microRNA in Drosophila GSC: summary (1)
34. microRNA in Drosophila GSC: summary (2)
35. microRNA function in hESCs
36. Dicer and Drosha knock-down
37. Lentiviral construct
38. Confirm knockdown by QPCR
39. mRNA profiling in KD cells
40. Drosha and Dicer KD hESC(H1)- super stem cells
41. Dicer KD cells have higher level of Oct4 protein
42. Division rate of Dicer and Drosha KD hESCs(H1)
43. Cell cycle status of the mutant hESCs(H1)
44. p21 protein is elevated in Dicer KD cells
45. microRNA profiling in H7, H1 and HSF6 cells
46. hESCs express a unique set of microRNAs
47. Downregulation of microRNAs upon differentiation
48. Differentiating vs. undifferentiated ES cells
49. Rescue of Dicer KD phenotypes
50. Summary
51. Acknowledgements

Topics Covered

• microRNAs
• Stem cells
• Stem cell cell cycle
• Stem cell checkpoints
• p21
• hESCs
• Stem cell self-renewing division
• Notch
• Niche

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on September 29, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Hannele Ruohola-Baker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.