• The Endoplasmic Reticulum: A Regulator of Calcium Homeostasis and Lipid Metabolism
• 32 min
  1. The endoplasmic reticulum: protein folding and control of calcium homeostasis

  • Prof. Marek Michalak
• 42 min
  2. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 53 min
  3. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 20 min
  4. Lipid metabolism and the endoplasmic reticulum

  • Prof. Luis Agellon
• The Endoplasmic Reticulum: A Protein Synthesis Factory
• 41 min
  5. Endoplasmic reticulum, protein synthesis and translocation machinery

  • Dr. Christopher Nicchitta
• 50 min
  6. Glycoprotein maturation and quality control in the endoplasmic reticulum

  • Prof. Daniel Hebert
• 44 min
  7. Membrane transport from the endoplasmic reticulum

  • Dr. Benjamin Glick
• 42 min
  8. The endoplasmic reticulum in plants

  • Dr. Jurgen Denecke
• The Endoplasmic Reticulum and the Secretory Pathway
• 60 min
  9. The endoplasmic reticulum: the unfolded protein response

  • Prof. Randal Kaufman
• Archived Lectures *These may not cover the latest advances in the field
• 45 min
  10. Physiology and pathophysiology of the calcium store in the endoplasmic reticulum of neurones

  • Prof. Alexei Verkhratsky
• 42 min
  11. The endoplasmic reticulum as a calcium gateway in programmed cell death

  • Prof. Nicolas Demaurex
• 47 min
  12. The endoplasmic reticulum as a most efficient antibody factory

  • Prof. Roberto Sitia

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Modulation of intracellular free Ca concentration
3. IP3 mediated calcium signals
4. Cytoplasm as an excitable medium
5. InsP3-mediated Ca release
6. IP3 receptors family
7. The InsP3R channel is a tetramer
8. Low resolution structures of the IP3 receptor
9. Atomic resolution structures for the IP3 receptor
10. Crystal structure modeling
11. Nuclear patch clamping
12. Single xenopus InsP3R channel
13. Current-voltage relation for InsP3R
14. InsP3R channel activity is regulated by [Ca]i
15. Biphasic manner of Ca regulation of InsP3R
16. Ca dependence of InsP3R: mean open duration
17. Ca dependence of InsP3R: mean closed duration
18. InsP3R channel activity is regulated by InsP3
19. How InsP3 gates the InsP3R channel
20. Na * Po
21. The number of channels activated in a patch (Na)
22. Channel recruitment enables dynamic response
23. The InsP3R channel conductance
24. Inevitable abrupt channel activity termination
25. Ligand concentration-dependence
26. Review
27. Similar channel behaviors in different species
28. Expression of rat type 3 InsP3R in Xenopus
29. Ca activation of types 1 and 3 InsP3R
30. Schematic diagram of Ca signaling elements
31. InsP3R-mediated Ca signaling
32. 3-D surface plot
33. 3-D surface plot of type 1 and 3 InsP3R
34. 3-D surface plot: cell at rest
35. 3-D surface plot: cell stimulated (type 1 channel)
36. 3-D surface plot: cell stimulated (type 3 channel)
37. Calcium induced calcium release
38. Both types 1 and 3 InsP3R propagate Ca release
39. Additional regulatory domains of the channel
40. Effect of free ATP on InsP3R Ca responses
41. The channel sensitivity to ATP changes
42. Is InsP3R localized within multi-protein complexes
43. CaBP1
44. Domain structures of CaBPs and calmodulin
45. Ca dependence of interaction of CaBP and InsP3R
46. The consequences of CaBP binding to the IP3R
47. CaBP1 binding activates InsP3R channel gating
48. Bcl-XL interacts with the IP3 receptor
49. InsP3R channel gating and Bcl-XL
50. Acknowledgements

Topics Covered

• The inositol trisphosphate receptor signaling system
• Dynamic properties of cytoplasmic Ca2+ signals
• Structural properties of the InsP3R
• Patch clamp electrophysiology of the InsP3R channel
• Permeation properties of the channel
• Channel gating regulation by Calcium and InsP3
• Channel mechanisms that account for graded Ca2+ release
• Isoform specific differences in channel gating
• Channel regulation by ATP and interacting proteins

Links

Series:

• Calcium Signaling I
• The Endoplasmic Reticulum

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. J. Kevin Foskett has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.