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Navigable Slide Index

1. Introduction
2. From gene to protein
3. Forward approach
4. Reverse approach
5. Reverse approach II
6. Chemical genetics
7. Affinity matrix
8. Forward approach of chemical genetics
9. SAR (structure-activity relationships)
10. Tagged library approach
11. Chemical genetics with tagged library
12. Brain development in zebrafish
13. Linker effect
14. Affinity matrix
15. Protein analysis
16. Reverse approach of chemical genetics
17. IgG binding small molecules
18. Limitations of linker tag
19. Internal tagging
20. Fluorescence compounds
21. Combinatorial library
22. Building blocks
23. Protocol details
24. Fluorescence library
25. Broad range of colors
26. Forward approach
27. Organelle targeting
28. Application of the method in reverse approach
29. In vitro screening
30. Amyloid beta staining in mice brain
31. HTS for specific protein binder?

Topics Covered

• Chemical genetics is defined as "genetic" study using "chemical" tools
• The key challenge in chemical genetics is target identification
• Tagged library has been developed and demonstrated in this lecture to address the problem

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Publication History

• Published on August 14, 2008
• Archived on August 13, 2020

Financial Disclosures

• Dr. Young-Tae Chang has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.