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1. Introduction
2. Peptide synthesis
3. Peptide theory of protein structure
4. Chemical structure of peptides
5. (Chemical) peptide synthesis
6. Epochs of chemical peptide synthesis
7. Classical solution synthesis
8. Protein-derived peptides
9. Solid phase peptide synthesis
10. Chemistry of SPPS
11. SPPS - reaction vessel
12. SPPS case study: cecropin A
13. SPPS case study: human beta-endorphin
14. Automated solid phase peptide synthesis
15. Impact of solid phase peptide synthesis
16. Fundamentals of SPPS
17. Synthesis takes place within the beads
18. 'Super-elastic' swelling of peptide-resin beads
19. Narrow-line NMR spectra of resin-bound peptides
20. Solubilizing effect of attachment to the resin
21. Functionalization of the resin support
22. Boc chemistry SPPS - protecting group scheme
23. Boc chemistry SPPS - cleavable linkers
24. Boc chemistry SPPS - protecting groups
25. Boc chemistry SPPS - chain extension
26. Boc chemistry SPPS - side reactions
27. Boc chemistry SPPS - deprotection and cleavage
28. Boc chemistry SPPS - HF apparatus
29. Boc chemistry SPPS - HF side reactions
30. Fmoc chemistry SPPS - protecting group scheme
31. Fundamentals of Fmoc chemistry SPPS
32. Fmoc chemistry SPPS - cleavable linkers
33. Chain extension and deprotection/cleavage
34. 'Difficult' peptide sequences in SPPS
35. The 'diffculty' is a property of the peptide chain
36. Examples of 'difficult' peptide sequences for SPPS
37. Effect of disrupting peptide aggregation
38. An optimized solid phase peptide synthesis
39. Boc SPPS case study: HIV-1 protease
40. Boc SPPS case study: DGK(1-25)-thioester
41. Optimized Fmoc SPPS
42. Fmoc SPPS case study: transmembrane peptide
43. Fmoc SPPS case study: FDD(50-95)-amide
44. Impact of modern analytical methods on SPPS
45. Preparation of large numbers of natural peptides
46. Extensions and applications of SPPS
47. Scope and limitations of SPPS
48. Byproducts accumulate as chain length increases
49. Synthesis of HIV-1 protease
50. Capability of routine chemical protein synthesis
51. Chemical protein synthesis
52. Synthesis of transmembrane peptide-thioesters
53. Impact of chemical ligation
54. Emil Fischer (1852-1919)
55. Total synthesis of human lysozyme
56. Impact of SPPS
57. Bruce Merrifield (1921-2006)

Topics Covered

• Chemical structure of peptides
• Chemical peptide synthesis
• Classical solution synthesis
• Solid-phase peptide synthesis
• Chemistry of SPPS
• Automated solid-phase peptide synthesis
• Impact of SPPS
• Fundamentals of SPPS
• Synthesis takes place within the beads
• 'Super-elastic' swelling of peptide-resin beads
• Boc chemistry SPPS
• Fmoc chemistry SPPS
• 'Difficult' peptide sequences in SPPS
• Optimized solid-phase peptide synthesis
• Impact on SPPS of modern analytical methods
• Extensions of SPPS & applications of the solid-phase principle
• Scope and limitations of SPPS
• Impact of chemical ligation
• Total synthesis of human lysozyme

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Publication History

• Published on August 14, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Stephen Kent has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.