• Structure of the blood-brain barrier
• 36 min
  1. The blood-brain barrier in Alzheimer’s disease

  • Dr. Anika Hartz
• 35 min
  2. Tanycytes allow a tight BBB in the median eminence

  • Prof. Esteban Rodriguez
  • Dr. Juan Luis Blazquez
  • Dr. Montserrat Guerra
• Nutrient transport
• 35 min
  3. Blood-brain barrier ion transport

  • Prof. Martha O'Donnell
• Peptides and proteins
• 37 min
  4. Ingestive peptides

  • Prof. William Banks
• 62 min
  5. Blood-brain barrier in health and disease

  • Prof. Thomas Davis
• Overcoming the blood-brain barrier obstacle
• 42 min
  6. Expression vs. function of ABC transporters at the blood-brain barrier

  • Prof. Jean-Michel Scherrmann
• 44 min
  7. The blood-brain barrier and CNS drug development

  • Dr. Danica Stanimirovic
• 34 min
  8. Transport and delivery of neurotrophic peptides and proteins across the BBB

  • Prof. Weihong Pan
• Diseases involving the blood-brain barrier
• 71 min
  9. Inflammation and immune cell entry to the central nervous system

  • Prof. Serge Rivest
• 42 min
  10. The blood-brain barrier in multiple sclerosis: a target for treatment?

  • Dr. Elga de Vries
• 24 min
  11. Ischemic blood-brain barrier and Alzheimer's amyloid plaques development

  • Prof. Ryszard Pluta
• Latest Developments in the Field
• 31 min
  12. MRI approaches for neurovascular imaging

  • Dr. Rick Dijkhuizen
• 33 min
  13. Brain-gut interactions in obesity 1

  • Prof. Weihong Pan
• 19 min
  14. Brain-gut interactions in obesity 2

  • Prof. Weihong Pan
• Archived Lectures *These may not cover the latest advances in the field
• 36 min
  15. Neurotrophins and the BBB

  • Prof. Weihong Pan
• 52 min
  16. Vasoactive peptides and the blood-brain barrier

  • Prof. Maria Deli
  • Prof. Bela Kis
• 42 min
  17. In vivo systems

  • Prof. Quentin Smith
• 46 min
  18. CNS-drug design

  • Prof. Quentin Smith
• 43 min
  19. Brain-to-blood efflux transport of endogenous compounds and its analogue drug at the blood-brain barrier

  • Prof. Tetsuya Terasaki
• 23 min
  20. Stroke and the BBB

  • Prof. Marilyn Cipolla
• 54 min
  21. Barrier mechanisms in the developing brain: mechanisms and misunderstandings

  • Prof. Norman Saunders
• 57 min
  22. Barrier mechanisms in the developing brain: protection or vulnerability?

  • Prof. Norman Saunders
• 43 min
  23. In vitro models of the blood-brain barrier

  • Prof. Pierre-Olivier Couraud
• 41 min
  24. Glucose transport across the blood-brain barrier

  • Prof. Luc Leybaert
• 53 min
  25. The blood-brain barrier and brain tumors

  • Dr. Olaf van Tellingen
• 37 min
  26. Cellular composition of the blood-brain barrier

  • Prof. N. Joan Abbott
• 48 min
  27. Features of mammalian CNS barrier systems

  • Prof. Conrad Johanson

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Lecture outline
3. The blood-brain-barrier
4. Cellular architecture of the BBB
5. Pericytes
6. Molecular characteristics of the BBB
7. Reference in vitro models of the BBB
8. Cultured brain microvascular endothelial cells
9. Phenotypic markers of brain endothelial cell
10. BBB in vitro models
11. Example of a co-culture system
12. In vitro permeability assay
13. Trans-endothelial electrical resistance (TEER)
14. In vitro and in vivo drug permeability
15. Drug permeability of various in vitro models
16. Immortalization of rat brain endothelial cells
17. Primary BMECs vs. immortalized cell lines
18. A new model of human BBB
19. Immortalization of human brain endothelial cells
20. hCMEC/D3: a non-transformed phenotype (1)
21. hCMEC/D3: a non-transformed phenotype (2)
22. hCMEC/D3: expression of junction proteins (1)
23. hCMEC/D3: expression of junction proteins (2)
24. Permeability characteristics of hCMEC/D3 cells
25. Efflux pupms: ABC transporters
26. hCMEC/D3: functionality of ABC transporters
27. The hCMEC/D3 cell line
28. The hCMEC/D3 model under flow
29. hCMEC/D3 in a DIV-BBB device
30. Hyperosmolar opening of BBB in DIV models
31. Radioactive sucrose permeability in DIV models
32. hCMEC/D3 in a DIV-BBB device: conclusions
33. Applications of in vitro models of the BBB
34. BBB: physiological and pathophysiological roles
35. In vitro BBB models and research axes
36. Leukocyte infiltration across the BBB
37. Response to cell adhesion and transmigration
38. ICAM-1and PECAM-1 signaling
39. Tight junctions, receptors and transporters
40. Analysis of TJ proteomes in hCMEC/D3 cells
41. Multiple reaction monitoring (MRM)
42. Analysis by quantitative mass spectrometry
43. Optimizing drug delivery to the brain
44. BBB in vitro models: conclusions, perspectives
45. Acknowledgments

Topics Covered

• Blood-brain barrier
• In vitro models of blood-brain barrier
• Blood-brain barrier permeability
• Brain endothelium and tight junctions
• Transporters at the blood-brain barrier

Links

Series:

• The Blood-Brain Barrier

Categories:

• Methods
• Neuroscience
• Pharmaceutical Sciences

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on March 1, 2008
• Archived on October 28, 2018

Financial Disclosures

• Prof. Pierre-Olivier Couraud has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.