• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Linear DNA in diverse bacteria
3. The "end replication" problem
4. Solving the end replication problem
5. Streptomyces
6. Replication pathway for adenovirus and Bacillus
7. Streptomyces DNA replication
8. A minimal pSLA2 replicon
9. pSLA2 replication - cis and trans acting regions
10. Linear DNA replication intermediate
11. Secondary structure of SLP2 plasmid end
12. Patching process mediated by terminal protein
13. The terminal proteins of Streptomyces
14. Comparison of seven TP sequences
15. Deoxynucleotidylation of TP
16. Recruitment of TP
17. Streptomyces summary
18. Borrelia
19. The segmented genome of Borrelia burgdorferi
20. Borrelia linear plasmids replication pathway (1)
21. Origin mapping by nascent strand analysis
22. Origin location in the linear chromosome
23. Plasmid maintenance proteins in B. burgdorferi
24. Minimal replicons
25. Regions required for lp17 replication
26. The telomere resolvase ResT
27. Borrelia linear plasmids replication pathway (2)
28. Purification of recombinant B. burgdorferi ResT
29. Assay for telomere resolution by ResT
30. The substrate
31. Three classes of telomere in B. burgdorferi
32. Minimal substrate requirements
33. Position of the cleavage site in telomeres
34. How does DNA cleavage and ligation occur?
35. ResT action mechanism
36. Alignment of telomere resolvases
37. Features of telomere resolvases
38. How does hairpin formation occur?
39. Alignment of hairpin binding modules with ResT
40. A composite active site and telomere resolution
41. A composite active site
42. Genome plasticity in linear Borrelia plasmids
43. Sequence scrambling in linear plasmids
44. Telomere exchanges
45. DNA rearrangements in B. burgdorferi
46. ResT reversal using a plasmid substrate
47. ResT-mediated telomere fusions
48. Sequence scrambling near telomeres
49. Reversal of telomere resolution
50. Conclusions - genome plasticity and ResT

Topics Covered

• The end replication problem and solutions
• Replication of linear DNA in Streptomyces species using terminal proteins
• Replication of linear DNA in Borrelia species using telomere resolution
• Genome plasticity in Borrelia resulting from reversal of telomere resolution

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008
• Archived on January 31, 2024

Financial Disclosures

• There are no commercial/financial matters to disclose.