• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Rolling-circle replicating (RCR) plasmids
3. Characteristicts of RCR plasmids
4. Functional organization of RCR plasmids
5. A model for plasmid rolling-circle replication
6. Replication in plasmids carrying two pT181 dsos
7. DNA sequence and structure of the pT181 origin
8. Initiator proteins of RCR plasmids (1)
9. Initiator proteins of RCR plasmids (2)
10. DNA nicking-closing activity of RepC
11. Domain structure of RepC initiator - pT181 plasmid
12. Conservation of the active tyrosine residue
13. DNA binding domains of pT181 family initiators
14. Replication origin of the pT181 family plasmids
15. Rep-DNA specificity
16. Role of individual monomers of the Rep protein
17. Purification of RepC heterodimers
18. SDS-PAGE analysis of purified RepC heterodimers
19. Division of labor between the two monomers
20. PcrA helicase
21. Role of the PcrA helicase in RC replication
22. Conversion of ss to ds DNA
23. General features of single-strand origins (SSOs)
24. Types of single strand origins of replication
25. SsoA origins of RC plasmids
26. Synthesis of primer RNAs from plasmid ssoA
27. Binding of staphylococal RNAP to pLS1/pE194
28. Binding of RNA polymerase to the ssoU
29. ssoA sequences of pLS1 and pE194 plasmids
30. pUB110/pMV158 ssoU
31. Model of lagging-strand synthesis from ssoA
32. Lagging strand replication - conclusions
33. Important unresolved issues

Topics Covered

• Rolling circle replicating (RCR) plasmids
• Functional organization of rolling-circle replicating plasmids
• Model for plasmid rolling-circle replication
• Initiation and termination of replication in plasmids carrying two pT181 dsos
• DNA sequence and structure of the pT181 origin
• Initiator proteins of RCR plasmids
• Comparison of the replication origins of the plasmids of pT181 family
• Rep-DNA specificity
• Role of individual monomers of the Rep protein
• Purification of RepC heterodimers
• PcrA helicase
• Role of PcrA helicase in plasmid RC replication
• Conversion of ss to ds DNA
• Single strand origins of replication
• Lagging-strand replication

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Saleem Khan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.