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1. Gene silencing by polycomb complexes
2. Hox genes
3. PcG mutation results in homeotic phenotypes
4. Drosophila PcG genes
5. PcG proteins are conserved from fly to human
6. PcG during the fly life cycle
7. PcG maintains Hox genes silencing
8. Questions concerning PcG silencing
9. Covalent modifications in histones
10. Histone methylation
11. Multiple histone methyltransferases in HeLa cells
12. Finding methyltransferases in HeLa cells
13. HMTase complex
14. Hox genes importance
15. EED-EZH2 complex
16. H3-K27 methylation
17. Loss of E(Z) function
18. Ubx gene silencing requirements
19. ESC-E(Z) function in S2 cells
20. E(Z) function in the wing discs
21. Pc binds to H3 through its chromodomain
22. Structural basis for binding of Pc to H3
23. Proposed model of gene silencing
24. PRC1 role in gene silencing?
25. Study in yeast
26. Establishment of an assay for E3 ligase activity
27. Detection of nucleosomal-specific E3 activities
28. H2A-K119 Ub-ligase
29. H2A-Ub ligase activity purification
30. Identity of the PRC1 complex components
31. Finding the catalytic subunit
32. H2A ubiquitination contribution?
33. dRing colocalize with ubH2A
34. Results of depletion of dRing by RNAi
35. The E3 ligase defective Sce33M2 mutant
36. Conclusions
37. How H2A ubiquitylation causes gene silencing?
38. Recruitment of E(Z) & PRC1 to PRE
39. Pho & Phol association with PRE
40. Role of Pho and Phol
41. Results (1)
42. Results (2)
43. Results (3)
44. Results (4)
45. Model for PcG silencing process
46. The diverse functions of the PcG complexes

Topics Covered

• Discovery of the Polycomb group (PcG) of proteins
• Role in embryogenesis
• Species conservation
• Latest developments in understanding molecular mechanism of gene silencing
• Evidence on hierarchical recruitment

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Series:

• Eukaryotic Gene Regulation

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on July 30, 2015

Financial Disclosures

• Prof. Yi Zhang has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.