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1. Introduction
2. The nucleosome
3. Histone post-translational modifications
4. Histones during transcriptional activation
5. Site specific recombination at the POH5 locus
6. Linking difference due to core particles removal
7. Chromatin remodeling
8. Measurment of relative nucleosome number
9. Nucleosomes retained on activated promotor
10. Nucleosomes are retained with differet probabilities
11. Results of limit nuclease and topology analysis
12. Why fractional value?
13. Two possible mechanisms of nucleosome removal
14. Reversing the order of activation and recombination
15. Results of the order reversing
16. Nucleosome removal: experimnetal results
17. Nucleosome removal: conclusions
18. Histone octamer transfer by the RSC
19. Assembly of transcription machinery
20. RNA polymerase II underlines the central dogma
21. RNA pol-II transcription machinery
22. Regulation of RNA pol II transcription
23. Conserved Mediator subunits
24. Mediator of transcriptional regulation
25. Structure of RNA pol II transcription machinery
26. 2-D protein crystallization
27. Obstacles to x-ray structure determination of pol II
28. Data on structure of pol II
29. Location of atoms of pol II
30. Polypeptide chains of pol II
31. Cleft of pol II
32. The nucleic acids in the transcription complex
33. The clamp
34. Structure of transcribing complex
35. Zoom-in on active center
36. Nucleotide addition cycle
37. Bridge helix structure
38. Surface representaion of the entire pol II
39. Upstream end of the DNA-RNA hybrid
40. Fork loops 1, rudder and lid
41. Transcriptions proteins interactions
42. Pol-II - TFIIB complex: N-termianl domain
43. TFIIB finger domain penetrates the cleft
44. B-finger and DNA-RNA hybrid
45. TFIIB stabilizes an initial transcribing complex
46. TFIIB-transcript competition
47. Pol-II-TFIIB complex: C-terminal domain
48. Structure of C-terminal domain of TFIIB
49. TBP structure
50. DNA bending along pol II surface
51. DNA bending in surface representation
52. Complete transcriptional initiation complex
53. Mechanism of initiation of pol II transcription
54. Modular structure of mediator
55. Conclusions

Topics Covered

• Chromatin remodeling for transcription
• The RNA polymerase II transcription machinery
• Mediator of transcriptional regulation
• Atomic structure of RNA polymerase II and of a transcribing complex
• Structural basis of ribonucleotide addition and transcript elongation
• Structure of a complete pre-initiation complex

Links

Series:

• From DNA to Proteins

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on April 17, 2016

Financial Disclosures

• Prof. Roger Kornberg has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.