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Printable Handouts
Navigable Slide Index
- Introduction
- Louis Pasteur axiom
- Random mutagenesis
- Strategies
- Single template
- Synthetic oligonucleoite site directed mutagenesis
- Bias
- MAX codons
- MAX primers
- Random mutagenesis of an entire gene
- Mutagenesis due to PCR error
- Random insertion/deletion mutagenesis (RID)
- Recombination based approaches
- DNA shuffling approaches
- StEP - staggered extension process
- Random chimeragenesis on transient templates
- Synthetic shuffling
- ADO - assembly of designed oligonucleotides
- Non-homologous recombination strategies
- Incremental truncation for hybrid enzyme (ITCHY)
- ITCHY / DNA shuffling combination
- SHIPREC
- Conclusions
Topics Covered
- Random mutagenesis
- Strategies
- Single template: oligonucleotide-based and PCRbased strategies
- Recombination-based strategies
- Bias
- MAX codons
- Random mutagenesis of an entire gene
- Mutagenesis due to PCR error
- Non-homologous recombination strategies
Talk Citation
Kaplan, A. (2007, October 1). Understanding protein variability through functional analysis of random mutagenesis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 16, 2024, from https://doi.org/10.69645/REEV4891.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Andrew Kaplan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
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