Printable Handouts

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Navigable Slide Index

1. Introduction
2. Louis Pasteur axiom
3. Random mutagenesis
4. Strategies
5. Single template
6. Synthetic oligonucleoite site directed mutagenesis
7. Bias
8. MAX codons
9. MAX primers
10. Random mutagenesis of an entire gene
11. Mutagenesis due to PCR error
12. Random insertion/deletion mutagenesis (RID)
13. Recombination based approaches
14. DNA shuffling approaches
15. StEP - staggered extension process
16. Random chimeragenesis on transient templates
17. Synthetic shuffling
18. ADO - assembly of designed oligonucleotides
19. Non-homologous recombination strategies
20. Incremental truncation for hybrid enzyme (ITCHY)
21. ITCHY / DNA shuffling combination
22. SHIPREC
23. Conclusions

Topics Covered

• Random mutagenesis
• Strategies
• Single template: oligonucleotide-based and PCRbased strategies
• Recombination-based strategies
• Bias
• MAX codons
• Random mutagenesis of an entire gene
• Mutagenesis due to PCR error
• Non-homologous recombination strategies

Links

Series:

• Protein Epidemiology

Categories:

• Biochemistry
• Cell Biology
• Methods

Talk Citation

Kaplan, A. (2007, October 1). Understanding protein variability through functional analysis of random mutagenesis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved May 9, 2025, from https://doi.org/10.69645/REEV4891.
Export Citation (RIS)

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Andrew Kaplan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.