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1. Introduction
2. Talk outline
3. Virus isolation
4. Animal hosts
5. Growth of virus on embryonated eggs
6. Cell culture
7. Primary cell culture
8. Subculture of primary cultured cells
9. Cell strains and cell lines
10. Cell transformation
11. Comparison of cultured cell types
12. Cultured cell morphologies
13. Recognition of virus infection in culture
14. Cytopathic effect: morphological changes
15. Cytopathic effect: measles
16. Cytopathic effect: Vaccinia
17. Cytopathic effect: inclusion bodies
18. Hemabsorption
19. Hemabsorption micrograph
20. Talk outline: assay of viruses
21. Biological assays of viruses
22. Plaque formation
23. Plaque micrograph
24. Plaque assay
25. Plaque assay photograph
26. Focus formation
27. Focus formation photograph
28. Endpoint titration
29. Physical assays of viruses
30. Hemagglutination
31. Hemagglutination test
32. Hemagglutination test: Influenza virus
33. Direct particle count
34. EM of direct particle count
35. Comparison of quantitative assays
36. One step growth experiment
37. Example of one step growth experiment
38. Talk outline: virus genetics
39. Virus genetics principles
40. Scope of virus genetics
41. Clinical significance of virus genetics
42. Mutation in viruses
43. Mutant phenotypes
44. Host range virus mutant
45. Host range subtypes
46. Engineered host range mutants
47. Temperature sensitivity
48. Temperature sensitive mutants
49. Example of temperature sensitivity
50. Antiviral drugs and virus genetics
51. Uses of drug resistance/dependence
52. Example of drug resistance
53. Example of drug dependence
54. Recombination and reassortment
55. DNA virus recombination
56. RNA virus recombination - copy choice
57. Segmented RNA virus reassortment
58. Reverse genetics
59. Summary and perspectives

Topics Covered

• Virus cultivation
• Virus isolation
• Animal hosts
• Cell culture
• Recognition of virus infection in culture
• CPE: morphological changes
• Hemadsorption
• Biological assay of viruses
• Plaque assay
• Focus assay
• Endpoint method
• Physical assay of viruses
• Hemagglutination
• Direct particle count
• Virus genetics
• Genetic principles
• Mutation
• Engineered host range
• Temperature sensitive mutants
• Antiviral drugs
• Uses of drug resistance/dependence
• Recombination and reassortment
• Reverse genetics

Links

Series:

• Viruses and Viral Diseases

Categories:

• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on April 29, 2020

Financial Disclosures

• Prof. Richard Condit has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.