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Printable Handouts
Navigable Slide Index
- Introduction
- Amniotic fluid and placenta
- Possible source for alternate stem cells?
- Amniotic fluid and placental derived stem cells
- 400 human amniotic fluid and placental samples
- Clonality confirmed by Southern blot
- Preservation of telomere length
- Cells had markers consistent with ES
- Cells had markers consistent with adult cells (1)
- Cells had markers consistent with adult cells (2)
- AFS cells show high similarity to ES and iPS
- Isolated cells after 250 population doublings
- AFS costimulatory molecule expression
- hAFS cells inhibit T-cell activation in vitro
- The cells are able to form embryoid bodies (EB)
- Embryoid bodies microscopy
- EB show the presence of 3 germ layers (1)
- EB show the presence of 3 germ layers (2)
- Immunomodulation
- Regenerative medicine application of AFS
- Fat differentiation
- Bone differentiation of human AFS cells
- Application of bone derived cells - printer
- Implantation of bone structure in rodent model
- Cartilage differentiation
- Cardiac differentiation
- How cardiac differentiation actually works
- Co-culture of NRCs with hAFS cells
- Co-culture of calcein preloaded NRCs with hAFS
- Endothelial differentiation
- Liver differentiation
- Lung differentiation
- hAFSC express endogenous PDPN
- Clara cells damage through naphthalene injury
- hAFSC did not show fusion
- Amniotic fluid stem cells in the kidney
- AFSC for renal therapy
- Kidney injury model
- Functionality of kidney injury model
- Necrotizing enterocolitis (NEC)
- NEC model
- 72 hrs post-injection (NEC model)
- PCR analysis
- EGFP amplification on genomic DNA
- Intestinal macroscopic assessment
- Intestinal microscopic assessment
- AFSC display hematopoietic activity
- Amniotic fluid & placental stem cells: clinical utility
- Summary
- Publication history
- Acknowledgements
Topics Covered
- Introduction to the amniotic fluid and placenta
- Collecting stem cells from the amniotic fluid or placenta
- Cell markers in amniotic fluid or placenta derived stem cells
- Amniotic fluid or placenta derived stem cells are able to form embryoid bodies
- Uses of amniotic fluid or placenta derived stem cells: Immunomodulation
- Uses of amniotic fluid or placenta derived stem cells: Regenerative medicine
- Amniotic fluid or placenta derived stem cells can be differentiated into a variety of cell types
- Amniotic fluid stems cells in the kidney
- Necrotizing enterocolitis; a model and treatment with amniotic fluid stems cells
- Clinical uses of amniotic fluid or placenta derived stem cells
Links
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Therapeutic Areas:
Talk Citation
Atala, A. (2014, March 5). Stem cells derived from amniotic fluid and placenta [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 11, 2024, from https://doi.org/10.69645/KMEV7099.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Anthony Atala, Stock Shareholder (Self-managed): Plureon, Inc.
A selection of talks on Gynaecology & Obstetrics
Transcript
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0:00
This is Anthony
Atala from the Wake Forest Institute
for Regenerative Medicine in
Winston-Salem, North Carolina.
And I will be discussing the
topic of stem cells derived
from amniotic fluid and placenta.
0:16
The amniotic fluid is the fluid
that surrounds the developing embryo
and fetus, and the placenta
is a tissue that surrounds
both the developing embryo and
fetus as well as the fluid.
Interestingly, both of these
sources, the fluid and the tissue,
are present all the way until the
time that the baby is delivered.
0:42
Both the amniotic
fluid and the placenta
can be obtained any time
after a certain period,
during gestation, all
the way through term.
Amniocentesis is a procedure
where amniotic fluid gets obtained
anywhere between 14 weeks of
gestation all the way through term.
And that is where we
obtain the amniotic fluid.
Chorionic villus sampling is
a procedure where we obtain
a small piece of a placenta any
time between 12 weeks through term.
Both techniques are widely accepted
methods for the prenatal diagnosis
of the fetus or the
embryo, and it can
be done all the way
through the time of birth.
It is well known that both
amniotic fluid and the placenta
are full of the
developing fetus cells.
However, what we postulated about
10 years ago, whether these sources
could in fact be a possible
source for stem cells.