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1. Introduction
2. Talk outline
3. Definition
4. Standardisation of the technique
5. Methods of sputum induction and processing
6. Facilities and instruments required
7. Sputum induction method
8. Alternative method of sputum induction
9. Key points (1)
10. Treatment with salbutamol and saline
11. Sputum induction shouldn't be repeated frequently
12. Repeated inductions
13. Sputum composition changes
14. Interferences among sputum induction
15. Methods of sputum processing
16. Characteristics of a sputum sample
17. Instruments needed to evaluate a sample
18. Two methods for processing sputum (1)
19. Two methods for processing sputum (2)
20. Sputum processing with DTT
21. Processing sputum samples
22. Key points (2)
23. Comparison of two methods of processing (1)
24. The methods are not interchangeable
25. Safety
26. Safety and reproducibility of sputum induction
27. Key points (3)
28. Pretreatment with a short-acting Beta 2-agonist
29. Predictors of excessive bronchoconstriction
30. No reports of death or hospital admission
31. Full resuscitation equipment should be available
32. Reproducibility
33. Reproducibility between sample
34. Validity and application in asthma
35. Comparison with other techniques
36. Comparing induced sputum and other methods
37. A correlation between eNO & sputum eosinophils
38. Eosinophil count and exhaled breath condensate
39. Comparing methods - summary
40. Reference values
41. Induced sputum cellularity
42. Differential cell count - normal values
43. Induced sputum cells in healthy controls
44. The influence of age
45. Disease vs. healthy
46. Comparison of two methods of processing (2)
47. Sputum eosinophil counts in asthma
48. Controlled vs. not controlled asthma
49. Sputum neutrophil counts in asthma
50. Sensitivity to risk factors or treatment
51. Analysis of cellular and biochemical constituents
52. Effects of prednisone on airway inflammation
53. Inhaled steroids and induced sputum eosinophils
54. Effects of anti-IgE omalizumab on inflammation
55. Targeted therapy with anti-IL5 in asthma
56. Eosinophils predicts response to corticosteroids
57. Clinical use
58. Asthma exacerbations and eosinophil counts
59. A randomised controlled trial
60. A randomised controlled trial - eosinophil count
61. A randomised controlled trial - exacerbations
62. Summary (1)
63. Summary (2)

Topics Covered

• Induced Sputum: facilities and procedure
• Induced Sputum as a biomarker of airway disease in asthma
• Comparison with other techniques
• Reference values and comparison between healthy and patients
• Induced sputum in asthma: clinical implications

Links

Series:

• Advances in Asthma

Categories:

• Cell Biology
• Clinical Practice
• Methods

Therapeutic Areas:

• Immunology & Inflammation
• Respiratory Diseases

Talk Citation

Publication History

• Published on January 17, 2013

Financial Disclosures

• Prof. Antonio Spanevello has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.