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Welcome to this lecture
on cell culture basics,
providing an overview of
cell culture techniques,
their purposes in research,
and the differences
between primary
and continuous cell lines.
We will discuss essential
requirements for cell growth,
including media composition,
environmental conditions,
and the importance of sterility.
The core principles
and practices of
aseptic technique
will be outlined,
along with the key
equipment used
in cell culture laboratories.
Finally, we will touch on
recent automation trends
while emphasizing
foundational practices
Cell culture
involves growing cells outside
their original organism in
a controlled environment.
This technique is widely used in
biological research,
biotechnology and medicine.
Its main purpose is to study
cell behavior,
understand diseases,
test drug candidates,
and produce
biological products like
vaccines and
therapeutic proteins.
Cultured cells
enable researchers
to observe cellular responses,
manipulate genes,
and model disease,
but require careful planning
and precise technique.
There are two main
categories of cell cultures,
primary and continuous
immortalized cell lines.
Primary cultures are derived
directly from tissues like
kidney or skin and
retain many characteristics
of their origin,
but have a limited lifespan,
often ceasing division after
a set number of passages,
the Hayflick limit.
Continuous cell lines can
proliferate indefinitely,
either naturally as
with tumor derived
hela cells or via
laboratory transformation.
