• Introduction
• 38 min
  1. DNA methylation during development

  • Prof. Howard Cedar
• The Nuts and Bolts of DNA Methylation
• 54 min
  2. DNA methylation patterns in mammals

  • Prof. John Greally
• 31 min
  3. Proteins that bind methylated DNA

  • Dr. Pierre-Antoine Defossez
• DNA Methylation and Normal Physiology
• 25 min
  4. Genomic imprinting

  • Prof. Marisa Bartolomei
• 32 min
  5. DNA methylation and stem cells

  • Dr. Alexander Meissner
• DNA Methylation and the Pathophysiology of Disease
• 54 min
  6. DNA methylation changes in aging cells and tissues

  • Prof. Jean-Pierre Issa
• 61 min
  7. DNA methylation in cancer

  • Prof. Paula Vertino
• 29 min
  8. Epigenomics of complex disease

  • Prof. Art Petronis
• 41 min
  9. DNA methylation, inflammation and cancer

  • Prof. Gerd Pfeifer
• Clinical Applications of DNA Methylation Research
• 41 min
  10. DNA methylation as a tumor marker: implications for diagnosis, early detection and risk assessment

  • Prof. Steve Belinsky
• 46 min
  11. Reversing DNA methylation pharmacologically

  • Dr. Allen Yang

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Talk outline
3. Epigenetic
4. Cytosine methylation
5. The fifth base: 5-methyl-cytosine
6. Discovery of 5-methyl-cytosine in vertebrates
7. Confirmation of 5-methyl-cytosine in vertebrates
8. DNA methylation - history
9. DNA methylation establishment and maintenance
10. Mammalian Dnmts are essential
11. Cytosine methylation in mammals
12. How is cell types diversity created and maintained
13. Potential of differentiated cells is more restricted
14. Nuclear transplantation experiments
15. DNA methylation: pluripotent vs. unipotent cell
16. The epigenome changes during differentiation
17. Genomic distribution of DNA methylation
18. Recent progress in genome-scale technologies
19. Categories for DNA methylation profiling
20. Sanger sequencing vs. Illumina sequencing
21. Bisulfite sequencing
22. Illumina Infinium
23. Reduced representations allow enrichment of CpG
24. High throughput reduced representation
25. meDIP-seq
26. MBD-capture-seq
27. DNA methylation in ES cells
28. Embryonic stem cells
29. Methyl-C levels in ES cells and tissues
30. CpG methylation levels are bimodal
31. CpG methylation levels correlate with CG density
32. Promoter methylation correlates with H3K4me3
33. Loss of Dnmt1 causes global demethylation
34. ES cells remain viable in low methylation levels
35. Female ES cells show global hypomethylation
36. The first human DNA methylation map (1)
37. The first human DNA methylation map (2)
38. Non-CpG methylation lost in absence of Dnmt3
39. DNA methylation during ES cell differentiation
40. Dynamic epigenetic changes
41. Inverse correlation of H3K4 and DNA methylation
42. Hypermethylation not recapitulated in vivo
43. DNA methylation in somatic cells
44. DNA methylation in somatic cells - recent data
45. Perspective
46. Perspective: human epigenome(s)
47. From reference genome to reference epigenome(s)
48. REMC assays
49. Epigenetic landscape
50. Acknowledgements

Topics Covered

• Stem cells
• Pluripotent stem cells are extremely valuable for disease modeling and regenerative biology
• DNA methylation
• Stable epigenetic modification

Links

Series:

• DNA Methylation

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on July 6, 2010

Financial Disclosures

• Dr. Alexander Meissner has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.