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This talk introduces
high performance liquid
chromatography, HPLC,
using it as a basis for
further exploration of
the fundamental principles and
components of high performance
liquid chromatography,
including separation
mechanisms and
key modes like reversed phase,
ion exchange, and
size exclusion.
We'll discuss important
analytical parameters such as
retention time and resolution
and how they're optimized
for better results.
Lecture will highlight
the broad applications of
HPLC in pharmaceuticals,
clinical diagnostics,
biomanufacturing and
beyond, demonstrating
its role as a powerful tool
in modern analytical science.
This essential
analytical technique is
widely used in scientific
labs to separate,
identify and quantify
components in complex mixtures.
HPLC has revolutionized areas
like drug analysis,
protein purification,
and clinical diagnostics
by exploiting
differing affinities
of molecules
for the stationary
and mobile phases,
allowing scientists to achieve
exceptional selectivity
and resolution.
Let's explore the principles and
main components of
an HPLC system.
In an HPLC instrument,
a high pressure pump moves
the mobile phase through
a column packed with
stationary phase material.
The sample is introduced via
an injector and travels through
the column where
separation occurs
based on properties like
polarity or charge.
Detectors, auto samplers,
solvent reservoirs,
and computerized systems
enhance precision,
reproducibility, and efficiency.
