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The following session will
cover light microscopy,
focusing on the fundamentals and
evolution of light microscopy,
highlighting its key techniques
and principle microscope types.
We will discuss how light
microscopes function,
the importance of
their components,
and approaches to
sample preparation.
The lecture will also examine
the diverse applications in
biology and material science,
along with the techniques
advantages and limitations.
Light microscopy is
an essential tool
in biological and
material sciences.
Using visible light and lenses,
light microscopes
let us visualize
structures far too small
for the naked eye,
sometimes down to
200 nanometers.
This technique reveals
the microscopic world,
allowing observation of cells,
tissues, microbes, and
even living processes.
Over time, light microscopy
has evolved into
various techniques,
meeting diverse research needs
and remaining a cornerstone
of laboratory science.
There are several principle
types of light microscopes,
each with specific benefits.
The most common is the
brightfield microscope,
ideal for stained or
pigmented specimens.
Transparent samples like
living cells require
specialized methods.
Phase contrast
microscopes enhance
contrast by using refractive
index differences,
allowing visualization
of live cells.
Dark field and
fluorescence microscopes
reveal fine structures
and track molecules.
Techniques like differential
interference contrast and
confocal microscopy
further improve
