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In this talk the focus is
fluorescence microscopy
supported by
coverage of the
fundamental principles
and components of
fluorescence microscopy,
highlighting how fluorescent
molecules provide
high contrast imaging of
specific cellular structures.
We will examine the types
of fluorophores used,
key applications such as
protein localization and
live cell imaging and
advanced techniques that
enhance resolution and detail.
Additionally, we will discuss
limitations like
photo bleaching,
signal overlap, and
strategies to overcome
these challenges.
Fluorescence microscopy is
a powerful imaging
technique that enables
visualization of specific
cell and tissue components
using fluorescent molecules.
Unlike conventional
light microscopy
which detects transmitted light,
fluorescence microscopy
uses fluorophores that will
absorb light at one wavelength
and emit it at a
longer wavelength.
This provides high
contrast images
allowing targeted
observation of proteins,
organelles, and
other structures.
The following discussion
explores the key components,
principles, applications and
limitations of
fluorescence microscopy.
The heart of a
fluorescence microscope
are several key
optical components.
The light source, often a
mercury or xenon lamp, laser,
or powerful LED emits
bright light in the
appropriate excitation range.
This light passes through
an excitation filter allowing
only specific wavelengths
to reach the specimen.
