• Chromosome Duplication
• 35 min
  1. Nucleosome assembly during DNA replication

  • Dr. Alain Verreault
• Spindle Assembly and Chromosome Segregation
• 54 min
  2. Chromosome bi-orientation in yeast

  • Prof. Mike Stark
  • Prof. Tomo Tanaka
• The Cell Cycle in Development and Cancer
• 62 min
  3. Mouse models to investigate cell cycle and cancer

  • Dr. Philipp Kaldis
• 47 min
  4. The Myc transcription factor network

  • Prof. Robert N. Eisenman
• Archived Lectures *These may not cover the latest advances in the field
• 24 min
  5. Geometric regulation of kinetochore orientation

  • Prof. Yoshinori Watanabe
• 54 min
  6. The spindle checkpoint

  • Dr. Kevin Hardwick
• 39 min
  7. Cleavage furrow formation and ingression during animal cytokinesis

  • Dr. Pier Paolo D'Avino
• 27 min
  8. Spindle movement and checkpoint control during mitosis in yeast

  • Prof. John Cooper
• 43 min
  9. The G2/M transition

  • Prof. Dr. René Medema
• 43 min
  10. Regulation of mammalian cell division by the chromosomal passenger complex

  • Dr. Susanne Lens
• 30 min
  11. Role and regulation of Cdk inhibitors in development and cancer

  • Prof. Martine Roussel
• 58 min
  12. Recombination and the formation of chiasmata in meiosis

  • Prof. Matthew Whitby
• 18 min
  13. Cell cycle: a complex network of signals regulating cell proliferation

  • Prof. Antonio Giordano
• 40 min
  14. Drug discovery and target validation in the p53 pathway

  • Prof. Sir David Lane
• 37 min
  15. START control in yeast

  • Prof. Curt Wittenberg
• 33 min
  16. The pRB/E2F pathway

  • Prof. Jacqueline Lees
• 35 min
  17. Cell cycle control by the ubiquitin system in mammals

  • Prof. Michele Pagano
• 47 min
  18. Replication licensing

  • Prof. Julian Blow
• 51 min
  19. Initiation of DNA replication

  • Prof. Bruce Stillman
• 48 min
  20. Regulation of replication fork progression and stability

  • Dr. Luis Aragón
• 27 min
  21. Sister chromatid cohesion: simple concept, complex reality

  • Prof. Douglas Koshland
• 50 min
  22. Mitotic chromosome condensation

  • Prof. Andrew Belmont
• 33 min
  23. Centrosome duplication and separation in animal cells

  • Prof. Andrew Fry
• 48 min
  24. The kinetochore as a target for the development of mitosis specific anti-cancer drugs

  • Dr. Tim Yen
• 34 min
  25. The DNA damage response

  • Dr. Vincenzo Costanzo
• 30 min
  26. Bipolar spindle assembly

  • Dr. Eric Karsenti

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Ensuring precise chromosome duplication
3. Activities required to control the duplication
4. Outline
5. The replication licence
6. Somatic cell fusion
7. Evidence for a replication licensing factor
8. Mcm2-7
9. The pre-replicative complex
10. Mcm4 in HeLa nuclei
11. Structure of Mcm2-7
12. Do Mcm2-7 provide the fork helicase?
13. The licensing reaction
14. Other pre-RC proteins
15. Origin recognition complex
16. Cdc6
17. Cdt1
18. Licensing chromatin with purified proteins
19. Licensing of replication origins
20. Cell cycle regulation of replication licensing
21. The licensing cycle
22. Result of mitotic CDKs inactivation in S. pombe
23. Pre-RC substrates of CDKs in yeast
24. The licensing cycle in S. cerevisiae
25. Cdt1 regulation in metazoa
26. Geminin - a metazoan licensing inhibitor
27. The licensing cycle in metazoan somatic cells
28. Licensing control in Xenopus eggs/embryos (1)
29. The licensing cycle in Xenopus embryos
30. Licensing control in Xenopus eggs/embryos (2)
31. Role of the ELYS-Mcm2-7 link
32. The licensing system and cancer
33. The 'MCM paradox'
34. Dormant origins
35. The license is lost on exit from the cell cycle
36. Mcm2-7 are down-regulated on cell cycle exit
37. Cervical carcinoma detection by Mcm5 staining
38. Licensing defects induced by oncogenes
39. Checkpoint in primary cell lines / cancer cell lines

Topics Covered

• Problems in ensuring complete genome replication
• Experiments revealing the existence of a replication licence
• The licensing reaction
• ORC, Cdc6, Cdt1 and Mcm2-7
• Cell cycle regulation of licensing
• Pre-RC substrates in yeast
• Regulation of licensing in animal cells by Cdt1 degradation and geminin inhibition
• Is there a licensing checkpoint?
• Replication licensing and cancer
• The 'MCM paradox' and dormant replication origins
• The licensing system as a potential anti-cancer target

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on April 30, 2009
• Archived on May 28, 2025

Financial Disclosures

• Prof. Julian Blow has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.