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- Co-ordination of G1 Progression
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37 min
1. START control in yeast- Prof. Curt Wittenberg
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33 min
2. The pRB/E2F pathway- Prof. Jacqueline Lees
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35 min
3. Cell cycle control by the ubiquitin system in mammals- Prof. Michele Pagano
- Chromosome Duplication
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47 min
4. Replication licensing- Prof. Julian Blow
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51 min
5. Initiation of DNA replication- Prof. Bruce Stillman
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48 min
6. Regulation of replication fork progression and stability- Dr. Luis Aragón
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35 min
7. Nucleosome assembly during DNA replication- Dr. Alain Verreault
- Preparing for Mitosis
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27 min
8. Sister chromatid cohesion: simple concept, complex reality- Prof. Douglas Koshland
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50 min
9. Mitotic chromosome condensation- Prof. Andrew Belmont
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33 min
10. Centrosome duplication and separation in animal cells- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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30 min
11. Bipolar spindle assembly- Dr. Eric Karsenti
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54 min
12. Chromosome bi-orientation in yeast- Prof. Mike Stark
- Prof. Tomo Tanaka
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43 min
- Mitotic Exit and Cytokinesis
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39 min
14. Cleavage furrow formation and ingression during animal cytokinesis- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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34 min
15. The DNA damage response- Dr. Vincenzo Costanzo
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54 min
16. The spindle checkpoint- Dr. Kevin Hardwick
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27 min
17. Spindle movement and checkpoint control during mitosis in yeast- Prof. John Cooper
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43 min
18. The G2/M transition- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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62 min
19. Mouse models to investigate cell cycle and cancer- Dr. Philipp Kaldis
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18 min
20. Cell cycle: a complex network of signals regulating cell proliferation- Prof. Antonio Giordano
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40 min
21. Drug discovery and target validation in the p53 pathway- Prof. Sir David Lane
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30 min
22. Role and regulation of Cdk inhibitors in development and cancer- Prof. Martine Roussel
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48 min
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47 min
24. The Myc transcription factor network- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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58 min
25. Recombination and the formation of chiasmata in meiosis- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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24 min
26. Geometric regulation of kinetochore orientation- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- Ensuring precise chromosome duplication
- Activities required to control the duplication
- Outline
- The replication licence
- Somatic cell fusion
- Evidence for a replication licensing factor
- Mcm2-7
- The pre-replicative complex
- Mcm4 in HeLa nuclei
- Structure of Mcm2-7
- Do Mcm2-7 provide the fork helicase?
- The licensing reaction
- Other pre-RC proteins
- Origin recognition complex
- Cdc6
- Cdt1
- Licensing chromatin with purified proteins
- Licensing of replication origins
- Cell cycle regulation of replication licensing
- The licensing cycle
- Result of mitotic CDKs inactivation in S. pombe
- Pre-RC substrates of CDKs in yeast
- The licensing cycle in S. cerevisiae
- Cdt1 regulation in metazoa
- Geminin - a metazoan licensing inhibitor
- The licensing cycle in metazoan somatic cells
- Licensing control in Xenopus eggs/embryos (1)
- The licensing cycle in Xenopus embryos
- Licensing control in Xenopus eggs/embryos (2)
- Role of the ELYS-Mcm2-7 link
- The licensing system and cancer
- The 'MCM paradox'
- Dormant origins
- The license is lost on exit from the cell cycle
- Mcm2-7 are down-regulated on cell cycle exit
- Cervical carcinoma detection by Mcm5 staining
- Licensing defects induced by oncogenes
- Checkpoint in primary cell lines / cancer cell lines
Topics Covered
- Problems in ensuring complete genome replication
- Experiments revealing the existence of a replication licence
- The licensing reaction
- ORC, Cdc6, Cdt1 and Mcm2-7
- Cell cycle regulation of licensing
- Pre-RC substrates in yeast
- Regulation of licensing in animal cells by Cdt1 degradation and geminin inhibition
- Is there a licensing checkpoint?
- Replication licensing and cancer
- The 'MCM paradox' and dormant replication origins
- The licensing system as a potential anti-cancer target
Talk Citation
Blow, J. (2009, April 30). Replication licensing [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2025, from https://doi.org/10.69645/VQIX5620.Export Citation (RIS)
Publication History
- Published on April 30, 2009
Financial Disclosures
- Prof. Julian Blow has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
