• Bioorganic Chemistry and the Origins of Chemical Biology
• 48 min
  1. Perspectives on biological catalysis

  • Prof. Stephen Benkovic
• Nucleic Acids and Drug/Nucleic Acid Interactions
• 41 min
  2. The importance of nucleotide size in the chemistry and biology of DNA

  • Prof. Eric Kool
• 27 min
  3. Site-specifically modified ribosomal RNAs

  • Prof. Christine Chow
• 26 min
  4. Creation of tools for the investigation of oxidative damage to nucleic acids

  • Dr. Mel Bedi
• Protein Function and Cellular Signaling
• 41 min
  5. Chemical synthesis of proteins

  • Prof. Lei Liu
• 55 min
  6. Visualizing and manipulating phosphoinositide and phospholipid signaling

  • Prof. Glenn Prestwich
• Peptides, Small-Molecules and Chemical Diversity
• 41 min
  7. Chemical synthesis of peptides and peptide libraries

  • Prof. Victor Hruby
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  8. Chemical protein synthesis: total synthesis of proteins for biological research

  • Prof. Stephen Kent
• 57 min
  9. Solid phase peptide synthesis

  • Prof. Stephen Kent
• 39 min
  10. Mapping small molecule binding sites with phage display technology

  • Dr. Lee Makowski
• 32 min
  11. Tagged library for chemical genetics

  • Dr. Young-Tae Chang
• 30 min
  12. Covalently constrained alpha-helices

  • Dr. Paramjit Arora
• 34 min
  13. SICLOPPS: genetic system for production of backbone cyclized peptides

  • Dr. Sergey Savinov
• 35 min
  14. Probing protein-protein interactions with peptide libraries and arrays

  • Dr. Zhou Songyang
• 48 min
  15. Drug-DNA interactions of the mitomycins

  • Prof. Maria Tomasz
• 86 min
  16. Phosphoserine/threonine binding domains: molecular integrators of protein kinase signaling in cell cycle control and cancer

  • Dr. Michael Yaffe
• 46 min
  17. Quantitative proteomics

  • Prof. Ruedi Aebersold
• 50 min
  18. Bioorganic studies of vision

  • Prof. Koji Nakanishi
• 59 min
  19. Polydactyl zinc finger proteins: software and hardware for genomes

  • Prof. Carlos Barbas
• 56 min
  20. Biomimetic catalysis

  • Prof. Ronald Breslow

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. From gene to protein
3. Forward approach
4. Reverse approach
5. Reverse approach II
6. Chemical genetics
7. Affinity matrix
8. Forward approach of chemical genetics
9. SAR (structure-activity relationships)
10. Tagged library approach
11. Chemical genetics with tagged library
12. Brain development in zebrafish
13. Linker effect
14. Affinity matrix
15. Protein analysis
16. Reverse approach of chemical genetics
17. IgG binding small molecules
18. Limitations of linker tag
19. Internal tagging
20. Fluorescence compounds
21. Combinatorial library
22. Building blocks
23. Protocol details
24. Fluorescence library
25. Broad range of colors
26. Forward approach
27. Organelle targeting
28. Application of the method in reverse approach
29. In vitro screening
30. Amyloid beta staining in mice brain
31. HTS for specific protein binder?

Topics Covered

• Chemical genetics is defined as "genetic" study using "chemical" tools
• The key challenge in chemical genetics is target identification
• Tagged library has been developed and demonstrated in this lecture to address the problem

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Publication History

• Published on August 14, 2008
• Archived on August 13, 2020

Financial Disclosures

• Dr. Young-Tae Chang has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.